The research of LncRNA FGD5⁃AS1 promotes hepatocellular carcinoma progression through miR⁃873⁃5p/GTPBP4 axis
10.19405/j.cnki.issn1000-1492.2022.02.015
- Author:
Nuobei Zhang
1
;
Shen ′an Huang
1
;
Hao Shen
1
;
Xin Chen
2
Author Information
1. Dept of Gastroenterology,2 Dept of Nuclear Medicine , The Second Afiliated Hospital ofNanchang University, Nanchang 330006
2. Dept of Nuclear Medicine , The Second Afiliated Hospital ofNanchang University, Nanchang 330006
- Publication Type:Journal Article
- Keywords:
hepatocellular carcinoma;
LncRNA FGD5⁃AS1;
miR⁃873 ⁃5p;
GTPBP4
- From:
Acta Universitatis Medicinalis Anhui
2022;57(2):240-247
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the regulatory mechanism of the long noncoding FGD5⁃AS1 ( LncRNA FGD5⁃AS1) on hepatocellular carcinoma(HCC)progression.
Methods :The expression level of FGD5 ⁃AS1 was measured in tumor tissues and cell lines by RT⁃qPCR. CCK⁃8 , EdU , flow cytometry , wound healing and transwell chamber assays were performed to investigate the role of FGD5 ⁃AS1 in HCC cell proliferation , apoptosis , migration and invasion in vitro. At the molecular level , dual luciferase reporter and RNA pull down assays were performed to identify the interaction among FGD5 ⁃AS1 , miR⁃873 ⁃5p and GTPBP4.
Results :FGD5 ⁃AS1 was upregulated in HCC tissues and cells. Moreover, FGD5 ⁃AS1 knockdown suppressed HCC cell proliferation , migration and invasion , and induced apoptosis in vitro. Mechanistically , FGD5 ⁃AS1 directly bound to miR⁃873 ⁃5p and competitively inhibit its expression to increase the expression level of GTPBP4 in HCC cells. Finally , our findings indicated that the role of FGD5 ⁃AS1 was mediated by miR⁃873 ⁃5p GTPBP4 axis.
Conclusion :FGD5 ⁃AS1 promotes the proliferation , migration and invasion of HCC cells and inhibits apoptosis by regulating the miR⁃873 ⁃5p/GTPBP4 axis.
- Full text:2024120517192476526LncRNA_FGD5-A...4轴促进肝细胞癌发展的研究_章诺贝.pdf
