The effect and mechanism of pulmonary vascular dysfunction in prenatal hypoxic offspring
10.19405/j.cnki.issn1000-1492.2022.02.014
- Author:
Meng Zhao
1
;
Huan Li
1
;
Bingyu Ji
1
;
Ting Xu
1
;
Qinqin Gao
1
Author Information
1. Institute for Fetology, The First Hospital of Soochow University, Suzhou 215006
- Publication Type:Journal Article
- Keywords:
prenatal hypoxia;
pulmonary artery;
vasoconstriction;
vasodilation;
calcium channels
- From:
Acta Universitatis Medicinalis Anhui
2022;57(2):235-239
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the effect and mechanism of prenatal hypoxia on pulmonary artery vascular function in adult male offspring.
Methods : Sprague⁃Dawley pregnant rats were randomly divided into hypoxia group and control group. Pregnant rats in the hypoxia group were housed in the hypoxia chamber ( a mixture of 89. 5% nitrogen gas and 10. 5% oxygen) from gestational day 10th to 20th. Pregnant rats in the control group were fed under
normal oxygen. All animals were fed with standard rat food and tap water, and allowed to give birth naturally. After weaning , the male offspring were raised to 4 months old. Male offspring rats were anesthetized with chloral hydrate , and pulmonary artery was isolated for vascular function and molecular experiments.
Results :Compared with the control group , phenylephrine ( PE) Ⅳ induced contraction response in prenatal hypoxic pulmonary artery was decreased (P < 0. 001) . There was no difference in acetyl choline (ACH) Ⅳand sodium nitrate (SNP) Ⅳinduced diastolic responses between the two groups. Compared with control group , Cav1 . 2 protein (P < 0. 01) and mRNA levels (P < 0. 001) in hypoxia group were reduced.
Conclusion :Prenatal hypoxia can lead to a decreased PE⁃induced vasoconstriction response in adult male offspring , which is related to the down⁃regulation of Cav1. 2 expression in pulmonary artery.
- Full text:2024120517082105617孕期缺氧对子代肺动脉血管功能的影响及其机制_赵梦.pdf
