Supervillin regulates convergence and extension movements via Wnt/β⁃catenin pathway in zebrafish embryos
10.19405/j.cnki.issn1000-1492.2022.02.011
- Author:
Lizhu Hu
1
,
2
;
Chenggang Zhao
1
,
2
;
Junqi Fan
1
,
2
;
Haoran Yang
3
;
Shangrong Zhang
4
;
Xueran Chen
4
;
Zhiyou Fang
4
Author Information
1. ept of Health and Medical Technology, Hefei Institutes ofPhysical Science , Chinese Academy of Sciences , Hefei 230031
2. Science Island Branch , Graduate School , University of Science and Technology of China , Hefei 230026
3. Dept of Health and Medical Technology, Hefei Institutes ofPhysical Science , Chinese Academy of Sciences , Hefei 230031 Dept of Health and Medical Technology, Hefei Institutes ofPhysical Science , Chinese Academy of Sciences , Hefei 230031
4. Dept of Health and Medical Technology, Hefei Institutes ofPhysical Science , Chinese Academy of Sciences , Hefei 230031
- Publication Type:Journal Article
- Keywords:
Svil;
concentrated extension movement;
Wnt/β⁃catenin;
zebrafish
- From:
Acta Universitatis Medicinalis Anhui
2022;57(2):218-223
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the role and molecular mechanism of Supervillin in zebrafish embryo development.
Methods :Clustal Omega and DNAman software were used to analyze and compare the homology of amino acid sequences of Svil and SVIL in zebrafish and human. The expression pattern of Svila (subtype of Svil protein in zebrafish) during early embryonic development was analyzed by RT⁃PCR and in situ hybridization. Svila expression in zebrafish was inhibited by injecting morphinos ( MO) , and morphological changes of embryos were observed. The expression and nuclear localization of β ⁃catenin protein were detected by Western blot , and the expression of Wnt/β⁃catenin target gene was detected by RT⁃PCR.
Results :During the early embryonic development of zebrafish , Svila was maternally expressed and showed an upward trend with the development process. The expression of Svila was reduced by MO , and the development of zebrafish embryos was distorted and the body axis was bent , which might be related to the blocked movement of concentrated extension of embryos. Further studies showed that Svila expression affected β⁃catenin nuclear transport and Wnt/β⁃catenin signaling pathway activation.
Conclusion :Svila regulates the concentrated extension movement of zebrafish embryos by activating the Wnt/β⁃catenin signaling pathway.
- Full text:2024120516320657990Supervilin通过W...调控斑马鱼胚胎集中延伸运动_胡丽珠.pdf
