A study on the mechanism of Avilamycin intervention MTIF2 regulating ribosomal translation process to inhibit hepatitis B virus replication
10.19405/j.cnki.issn1000-1492.2022.02.008
- Author:
Kai Chang
1
;
Wanlin Na
1
;
Chenxia Liu
1
;
Zhongyong Jiang
1
;
Yanyan Wang
1
;
Hongxuan Xu
2
;
Jinlan Shen
1
;
Yuan Liu
1
Author Information
1. Dept of Clinical Laboratory, The General Hospital of Western Theater Command , Chengdu 610083
2. Biology Teaching and Research Group , Chengdu Experimental Foreign Language School , Chengdu 610083
- Publication Type:Journal Article
- Keywords:
avilamycin;
hepatitis B virus;
mRNA translation;
translation initiation factor;
regulatory mechanism
- From:
Acta Universitatis Medicinalis Anhui
2022;57(2):203-207
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the inhibitory effect and molecular mechanism of ribosomal translation factor inhibitor Avilamycin on hepatitis B virus replication.
Methods: Liver cancer Hep3B cells were treated with different concentrations of Avilamycin. Cell activity was detected by CCK⁃8 ; the apoptosis was detected by flow cytometry , and HBV⁃DNA、pgRNA、MTIF2、RPL10 gene expression level was detected by qPCR method. The HBsAg and HBeAg was detected by ELISA. The AFP was detected by chemiluminescence. Aspartate aminotransferase (AST) , alanine aminotransferase(ALT) , and alkaline phosphatase (ALP) proteins was detected by Biochemistry method.
Results :Avilamycin had no inhibitory effect on Hep3B cell proliferation and apoptosis. However, it could promote
cellular AST secretion , reduce AFP levels , and have less effect on ALP secretion. In Hep3B cells , Avilamycin promotes accumulation of pgRNA expression by intervening with MTIF2 and feedback upregulates mRNA expression of host RPL10 and MTIF2 genes. It can effectively reduce the HBsAg , HBeAg , and HBV - DNA levels.
Conclusion :Avilamycin can inhibit MTIF2 translation initiation , regulate the translation process of viral assembly protein by affecting translation initiation , and then inhibit hepatitis B virus replication.
- Full text:2024120516040211368阿维霉素干预MTIF2调控...乙型肝炎病毒复制的机制研究_常凯.pdf
