Immune checkpoint VISTA has a role in the modulation of immune response in septic mice
10.19405/j.cnki.issn1000-1492.2022.02.006
- Author:
Tianzhu Tao
1
,
2
;
Guorong Zhang
3
;
Xiaoming Yang
1
;
Qing Guo
4
;
Jun Liu
5
Author Information
1. Dept of Anesthesiology, Air Force Medical Center, Beijing 100142
2. Dept of Pain Medicine , Air Force Medical Center, Beijing 100142
3. Dept of Pain Medicine , Air Force Medical Center, Beijing 100142
4. Research Dept of Special Medical Center of Armed Police , Beijing 102613
5. Dept of Research , Air Force Medical Center, Beijing 100142
- Publication Type:Journal Article
- Keywords:
immune checkpoint;
sepsis;
inflammatory response;
apoptosis
- From:
Acta Universitatis Medicinalis Anhui
2022;57(2):193-196, 202
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To determine expression profile of VISTA on lymphocyte and the potential regulatory effects on immune response in a murine model of sepsis.
Methods :Expression profile of VISTA on T lymphocytes in the spleen and peripheral blood was examined. Mice wereintravenously injected with an agonistic VISTA mAb(MH5A) or isotype control , and then the survival rate , cytokine expression , bacterial burden and lymphocyte apoptosiswere determined.
Results :VISTA was substantially expressed on T cell from the spleen and peripheral blood , septic peritonitis did not induce significant changes in the expression profiles at 24h post surgery(P > 0. 05 in each comparison) . Treatment with MH5A improved the survival of septic mice(8/12 vs 2/12 , P = 0. 01) , accompanied by reduced lymphocyte apoptosis [(30 123 ± 6 400) vs (45 482 ± 8 652) , P = 0. 03] , decreased bacterial burden and lessened cytokines expression , such as TNF⁃α , IL⁃6 , IL⁃1β and IFN⁃γ(P < 0. 05 in each comparison) .
Conclusion:The present study identified VISTA as a novel immune checkpoint in the regulation of T cell apoptosis and inflammatory response during sepsis. VISTA agonism might offer a promising approach in the immunotherapy for sepsis.
- Full text:2024120511223151869免疫检查点VISTA分子对...小鼠免疫炎症反应的调节作用_陶天柱.pdf
