The effect of iucA gene deletion on the proliferation , adhesion , invasion and colonization ability of Uropathogenic E. coli
10.19405/j.cnki.issn1000-1492.2022.02.003
- Author:
Cong Deng
1
;
Jiale Kang
1
;
Meishuang Lin
1
;
Yanfen Lin
1
Author Information
1. Dept Of Clinical laboratory, The Second Afiliated Hospital of Guangzhou Medical University, Guangzhou 51026
- Publication Type:Journal Article
- Keywords:
iucA gene;
Escherichia coli;
urinary tract infection;
siderophore;
gene knockout
- From:
Acta Universitatis Medicinalis Anhui
2022;57(2):177-181
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the role of iron uptake⁃related gene iucA in the pathogenesis of Uropathogenic E. coli(UPEC) , UPEC CFT073 strain was used to construct the iucA gene deletion strain ΔiucA by λ Red homologous recombination technology , and the complemented strain C ⁃iucA was also constructed.
Methods :The proliferation rates of CFT073 , ΔiucA and C ⁃iucA in Luria⁃Bertani liquid medium and sterile urine were determined by measuring the absorbance at 600 nm. Cultured 5637 human bladder cancer epithelial cells confluent monolayers were incubated with CFT073 , ΔiucA and C ⁃iucA for adhesion and invasion ability assay. C57BL/6 mouse urinary tract infection model was constructed to assess colonization ability of CFT073 , ΔiucA and C ⁃iucA in murine bladder tissues.
Results :Data showed that CFT073 , ΔiucA and C ⁃iucA displayed the similar proliferation curves in LuriaBertani liquid medium(P = 0. 153) . The proliferation rate of △iucA in sterile urine was lower than that of CFT073 ( P = 0. 001) , while the proliferation rate of C ⁃iucA in sterile urine was significantly higher than that of ΔiucA (P = 0. 005) . △iucA demonstrated much lower adhesion and invasion ability compared with CFT073 ( P = 0. 007 , 0. 002) , and C ⁃iucA showed significantly higher adhesion and invasion ability than △iucA( P = 0. 046 , 0. 037) . The colonization ability of △iucA in murine bladder tissues was much lower than that of CFT073 and C ⁃iucA(P = 0. 002 , 0. 017) .
Conclusion :The results indicate iucA gene may contribute to the proliferation , adhesion , invasion and colonization ability of UPEC.
- Full text:2024120510290950071iucA基因缺失对尿路致病...黏附、侵袭和定植能力的影响_邓聪.pdf
