Effects of lanosterol synthase inhibitor on differentiation and apoptosis of KCs
10.19405/j.cnki.issn1000-1492.2022.02.002
- Author:
Li Liu
1
;
Yanan Gu
1
;
Mingcong Li
1
;
Yixuan Huang
1
;
Shengquan Zhang
1
Author Information
1. Center for Scientific Research of Anhui Medical University,Dept of Biochemistry and Molecular Biology, College of Basic Medicine , The First College of Clinical Medicine ,Hefei 230032
- Publication Type:Journal Article
- Keywords:
RO;
CH;
KCs;
cell differentiation;
cell apoptosis
- From:
Acta Universitatis Medicinalis Anhui
2022;57(2):173-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effects of RO or incubation with cholesterol ( CH) on the differentiation and apoptosis of KCs.
Methods :Ca2 + ( 1. 8 mmol/L) was added to KCs for 1 day after co - culture of RO alone or combined with CH in KCs for different time. The expression of Involucrin (INV) and Loricrin in KCs was analyzed by Western blot. After co⁃culture of RO alone or combination with CH in KCs for different time , the apoptotic changes of KCs cells were detected by flow cytometry and the expression of apoptotic proteins Bax and Bcl⁃2 were verified by Western blot.
Results :RO down⁃regulated the expression of Ca2 + induced differentiation marker protein INV , but weakly inhibited the expression of Loricrin , while CH showed no antagonistic effect on RO. RO induced
apoptosis of KCs cells in a time dependent manner. CH could antagonize the apoptotic effect of RO on KCs ; the expression of Bcl⁃2 and Bax was inhibited when RO was applied alone , CH could partially antagonize the inhibition effect of RO on Bcl⁃2 expression , but had no significant effect on Bax; however, RO reduced the ratio of Bcl⁃2/Bax in a time⁃dependent manner, and CH partially weakened the effect of RO on the ratio of Bcl⁃2/Bax.
Conclusion:RO may inhibit KCs differentiation and induce KCs cell apoptosis by down⁃regulating the expression of Loricrin and Bcl⁃2.
- Full text:2024120510225750363羊毛固醇合成酶抑制剂对KCs细胞分化和凋亡的影响_刘莉.pdf
