Research on CD147 inhibiting oxidative stress in prostate cancer cells
10.19405/j.cnki.issn1000-1492.2022.01.027
- Author:
Haiyue Xu
1
;
Zehao Li
1
;
Yongqi Han
1
;
Liguo Wang
2
;
Fang Fang
1
Author Information
1. Dept ofImmunology, College ofLaboratory Medicine ,Jilin Medical University,Jilin 132013
2. The Afiliated Hospital of Jilin Medical University,Jilin 132013
- Publication Type:Journal Article
- Keywords:
CD147;
oxidative stress;
prostate cancer;
PI3K/AKT
- From:
Acta Universitatis Medicinalis Anhui
2022;57(1):144-147, 152
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of CD147 on reducing hydrogen peroxide - induced oxidative stress injury in prostate cancer LNCaP cells.
Methods :The lentiviral system was used to establish a CD147 ⁃silencing prostate cancer cell model (LNCaP/shCD147 cells) and a negative control cell (LNCaP/Scramble cell) , and RT⁃qPCR was performed for verification. By detecting the activity of reactive oxygen species ( ROS) , superoxide dismutase (SOD) , glutathione peroxidase ( GSH⁃PX) and malondialdehyde ( MDA) in LNCaP/shCD147 and LNCaP/Scramble cells to verify the changes of oxidative stress and antioxidant enzymes in prostate cancer cells after silencing CD147 ; hydrogen peroxide( H2 O2 ) was added to the cells and the cell growth was detected by CCK⁃8 ; Western blot was used to detect the expression changes of nuclear factor E2 related factors (Nrf2) and heme oxygenase⁃1 (HO⁃1) to verify the relationship between the oxidative stress that occurs in prostate cancer cells after silencing CD147 and the PI3K/AKT signaling pathway.
Results :Successfully constructed a CD147⁃silencing prostate cancer cell model. Compared with LNCaP/Scramble cells , the expression of CD147 in mRNA was reduced (P <0. 01) . The results of oxidative stress showed that the content of ROS and MDA in cells increased after silencing CD147 (ROS , P < 0. 01 ; MDA , P < 0. 05) , while the activities of SOD and GSH⁃PX decreased(P < 0. 01) , indicating that after silencing CD147 , LNCaP/shCD147 cells undergo oxidative stress. In addition , with the increase of H2O2 concentration , the survival rate of LNCaP/shCD147 group cells decreased (P < 0. 01) . After inhibiting the PI3K/AKT signaling pathway , the expressions of Nrf2 and HO⁃1 in the LNCaP/shCD147 group were reduced (P < 0. 01) , indicating that CD147 inhibits the oxidative stress injury of prostate cancer cells through the PI3K/AKT pathway.
Conclusion :CD147 can reduce the oxidative stress damage of PCa cells , and its inhibitory mechanism
may be related to the PI3K/AKT signaling pathway.
- Full text:2024120316540069920CD147抑制前列腺癌细胞氧化应激作用_徐海月.pdf
