The preliminary study of the effects of chronic inflammatory pain on the gut flora of mice
- Author:
Xiaomin Wang
1
;
Peng Xu
1
;
Yuqi Wang
1
;
Jianye Zeng
1
;
Sichen Zhou
1
;
Chengzhi Xing
1
;
Xiangyu Hu
1
;
Yanan Wu
1
;
Yiruo Zhang
1
;
Hongjie Fan
1
Author Information
- Publication Type:Journal Article
- Keywords: high⁃thorough sequencing; chronic inflammatory pain; gut flora; diversity
- From: Acta Universitatis Medicinalis Anhui 2022;57(1):138-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective :This experiment was carried out to study the effects of chronic inflammatory pain on the gut flora of mice by 16S rRNA high⁃throughput sequencing.
Methods :Twelve specific pathogen free (SPF) C57BL/ 6J mice were randomly divided into CFA group and mock group , with 6 mice in each group. Chronic inflammatory pain model was established by intraplantar injection of CFA in the right posterior pelma of C57BL/6J mice. In the control group , normal saline was injected by intraplantar injection in the same position. Two weeks later, the mice were euthanized , and the feces in the colon were collected. The feces of two mice in the same group were mixed , detected and analyzed by 16S rRNA high⁃throughput sequencing technology.
Results :Compared with mock group , the abundance and diversity of gut flora in CFA group decreased. The abundance of Firmicutes and TM7 increased at the phylum level , the abundance of Aerococcus , Lactobacillus and Desulfovibrio increased significantly at the fami ⁃ ly and genus level , while the abundance of Psychrobacter, Prevotella , Oscillospira and Bifidobacterium decreased significantly compared to mock group. In addition , many biomarkers were found from the level of the phylum to the genus.
Conclusion :The gut microflora structure , especially the dominant flora , has changed significantly in mice with chronic inflammatory pain , which can provide basis for the treatment of microecological imbalance caused by chronic inflammatory pain and the improvement of patients ′ negative emotions through“ gut brain axis”. - Full text:2024120316463085573慢性炎性疼痛对小鼠肠道菌群的影响_王小敏.pdf
