Interventive effect of glycyrrhizin on silicotic fibrosis in mice
https://kns.cnki.net/kcms2/article/abstract?v=ifIT5_n5_Ge8mcAbha6f9IIuu1YTf2wc6v657o7q-HxGj0ObIxNI18tvd_-JTXteFRBhpxjAhHcEByHh5aR7vHE6XBX4Tr44VdwCQYCNxdCLjPWnKGwnCheTJGROSQfGALrGteA3xTPG9zGcoz7DwxdWTuZpSUU5aI9Ff1LctJToad1Ug0u6Fa_p4R11NLl2&uniplatform=NZKPT&language=CHS
- Author:
Jing Zhang
1
;
Yiming Guo
1
;
Enhong Li
1
;
Mengmeng Zhao
1
;
Jiajia Jia
1
;
Xiaohui Hao
1
,
2
;
Lingli Guo
1
,
2
;
Heliang Liu
1
,
2
Author Information
1. School of Public Health , North China University of Science and Technology, Tangshan 063210
2. Hebei Key Laboratory of Organ Fibrosis , Tangshan 06321
- Publication Type:Journal Article
- Keywords:
silicosis;
glycyrrhizin;
fibrosis;
transforming growth factor⁃β1
- From:
Acta Universitatis Medicinalis Anhui
2022;57(1):121-125
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of glycyrrhizin on the fibrosis of silica⁃treated mice.
Methods :C57BL/6 male mice were randomly divided into control group , silicosis model group and glycyrrhizin treatment group ,with 6 mice in each group. The pathological changes of lung tissues were observed by HE and Sirius red stai⁃ ning. Lung function indexes were detected by respiratory function instrument. The content of hydroxyproline in the lung tissues was detected by corresponding kit. The mRNA levels of monocyte chemotactic protein 1 (MCP⁃1) , fibronectin (FN) and alpha⁃smooth muscle actin ( α ⁃SMΑ) were detected by real⁃time fluorescent quantitative PCR. The number of leukocytes in the bronchoalveolar lavage fluid (BALF) was counted and the secretion of transforming
growth factor⁃β1 (TGF⁃ β1) in BALF was detected by ELISA.
Results :HE and Sirius red staining showed that the inflammatory cells and the collagen were accumulated in the lung tissue of mice in silicosis model group. After treatment with glycyrrhizin , the accumulation of inflammatory cells and the collagen was ameliorated. Compared with the control group , pause (PAU) and enhanced pause (Penh) increased in the model group (P < 0. 05) . Glycyrrhizin treatment improved the respiratory function in mice. Furthermore , glycyrrhizin also effectively reduced the increase in the content of hydroxyproline , the expression of MCP⁃1 , FN and α ⁃SMΑ mRNA , the number of leukocytes and the secretion of TGF⁃ β1 induced by silica treatment in mice (P < 0. 05) .
Conclusion :Glycyrrhizin can improve the pulmonary function and alleviate the fibrosis in mice with silicosis.
- Full text:2024120310370520625甘草甜素对小鼠矽肺纤维化的干预作用_张静.pdf
