Anti⁃tumor effct of ginsenosides in the treatment of chemotherapeutic drugs resistance induced by the expression of CLDN18⁃ARHGAP26 fusion
10.19405/j.cnki.issn1000-1492.2022.01.021
- Author:
Jing Li
1
;
Bo Xie
1
;
Hu Wang
1
;
Chensong Zhang
1
;
Jianguang Jia
1
;
Chengwu Pan
1
;
Jiachi Ma
1
Author Information
1. Dept of Oncology, The First Afiliated Hospital ofBengbu Medical College ,Bengbu 233004
- Publication Type:Journal Article
- Keywords:
ginsenoside;
CLDN18⁃ARHGAP26 fusion mutated gene;
epithelial⁃mesenchymal transition;
drug resistance
- From:
Acta Universitatis Medicinalis Anhui
2022;57(1):111-116
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the effect of chemotherapeutic drug resistance induced by CLDN18⁃ARH⁃GAP26 fusion mutation gene in gastric cancer cells , and to investigate the sensitization effect of ginsenoside in the
treatment of chemotherapeutic drug resistance caused by the expression of CLDN18⁃ARHGAP26 fusion mutation gene.
Methods :The side population (SP) cells and non⁃side population ( NSP) cells of gastric cancer cell line BGC⁃823 were labeled with immunomagnetic bead antibody , and the lentiviral vector with overexpression of CLDN18⁃ARHGAP26 fusion mutation gene was selected for transfection with NSP cells. qPCR was used to detect the mRNA levels of CLDN18⁃ARHGAP26 fusion mutation and ATP Binding Cassette Subfamily G Member 2 (ABCG2) . The expression of EMT⁃related proteins E ⁃Cadherin and Vimentin was detected by Western blot. The sensitivity of transfected cells to oxaliplatin was detected by CCK⁃8. The effect of ginsenoside on drug resistance of transfected cells was detected by CCK ⁃ 8 . The expression of E ⁃ Cadherin and Vimentin in transfected cells was detected by Western blot after ginsenoside treatment.
Results : qPCR detection showed that the expression of CLDN18⁃ARH⁃GAP26 fusion mutant gene in NSP cells transfected with overexpressed CLDN18⁃ARHGAP26 fusion mutant gene was significantly higher than that of the non⁃transfected group , and the expression of ABCG2 mRNA was significantly cells with over⁃expressed CLDN18⁃ARHGAP26 fusion mutation gene was lower than that in the non⁃transfected
transfected cells to oxaliplatin was lower than that in the non⁃transfected group. The survival rate of transfected cells sion of E ⁃Cadherin protein in the transfected cells treated with ginsenoside was higher than that in the untreated group , and the expression of Vimentin protein was lower than that in the untreated group.
Conclusion : Ginsenoside can reverse cell EMT transformation and oxaliplatin resistance induced by CLDN18⁃ARHGAP26 fusion mutated gene expression in gastric cancer tissues.
- Full text:2024120310130872886人参皂苷在治疗因CLDN1...疗药治疗过程中的抗肿瘤作用_李靖.pdf
