Effect of lentivirus mediated silencing of NIPBL gene on osteogenic differentiation of mouse bone marrow mesenchymal stem cells
10.19405/j.cnki.issn1000-1492.2022.01.020
- Author:
Dekun Jiang
1
;
Huirong Zhang
1
;
Jinyong Pan
1
;
Wenqing Ma
1
;
Hui Liu
1
;
Lili Dong
1
Author Information
1. Dept of Pediatrics , The First Afiliated Hospital of Shihezi University School of Medicine , Shihezi 832000
- Publication Type:Journal Article
- Keywords:
lentivirus;
NIPBL gene;
bone marrow mesenchymal stem cells;
osteogenic differentiation;
Cornelia de Lange syndrome
- From:
Acta Universitatis Medicinalis Anhui
2022;57(1):105-110
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the effect of lentivirus mediated silencing of NIPBL gene on osteogenic differentiation of mouse bone marrow mesenchymal stem cells ( BMSCs) .
Methods :The third generation C57 Mouse Bone marrow mesenchymal stem cells were divided into experimental group , negative control group and blank control group. The lentiviral vector was transfected into mouse bone marrow mesenchymal stem cells , the transfection results were observed by inverted fluorescence microscope , and the expression of NIPBL gene was detected by real- time PCR. The cells of each group were cultured by osteogenic induction. The alkaline phosphatase activity was 2 and RUNX⁃2.
Results :The expression of NIPBL mRNA decreased in the experimental group (P < 0. 05) . The activity of alkaline phosphatase in experimental group was lower than that in negative control group and blank control group (P < 0. 05) . The gene transcription and protein expression levels of OCN, BMP⁃2 and Runx⁃2 in experimental group were lower than those in negative control group and blank control group ( P < 0. 05 ) . Alizarin red staining results showed that the negative control group and blank control group had more red calcium nodules than the experimental group.
Conclusion :Lentivirus mediated silencing of NIPBL gene reduces the proliferation of mouse bone marrow mesenchymal stem cells , inhibits the expression of osteogenic differentiation related genes , and reduces the osteogenic differentiation ability.
- Full text:2024120310043414065慢病毒介导沉默NIPBL基...质干细胞成骨分化能力的影响_姜德坤.pdf
