Study on the material basis and molecular mechanism of Rhei Radix et Rhizoma-Persicae Semen combination in activating blood circulation and dispelling blood stasis based on efficacy experiments, network pharmacology and HPLC
10.16438/j.0513-4870.2024-0027
- VernacularTitle:基于功效实验-网络药理学-HPLC探讨大黄-桃仁配伍活血化瘀的物质基础及分子作用机制
- Author:
Lin ZHU
1
,
2
;
Ying LIU
1
,
2
;
Jie SHEN
1
,
2
;
Bo-rui LI
1
;
Ke-xin YUE
1
;
Xia SHEN
1
,
2
;
Fan PING
3
Author Information
1. College of Pharmacy, Shaanxi University of Traditional Chinese Medicine, Xixian New Area 712046, China
2. Engineering Research Centre for Application and Development of Chinese Herbal Medicine in the Qinling Mountains of Shaanxi Province, Xixian New Area 712046, China
3. Department of Pharmacy, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China
- Publication Type:Research Article
- Keywords:
Rhei Radix et Rhizoma-Persicae Semen;
blood stasis syndrome;
activating blood circulation and dispelling blood;
molecular mechanism;
functional substance of Chinese medicine
- From:
Acta Pharmaceutica Sinica
2024;59(7):2126-2134
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the effective substance group and molecular mechanism of Rhei Radix et Rhizoma-Persicae Semen combination (RRR-PS) in activating blood circulation and dispelling blood stasis were investigated by integrating efficacy experiments, network pharmacology and HPLC. The rat model of blood stasis syndrome was established, and the blood rheology index and coagulation four comprehensive evaluation were carried out. The results showed that compared with the model group, the whole blood viscosity, erythrocyte sedimentation rate and erythrocyte aggregation index of the rats in the RRR-PS group were significantly callback (P < 0.01). Network pharmacology found that RRR-PS combination exerted the effect of activating blood circulation and dispelling blood stasis by acting on calmodulin-1 (CALM1), nitric oxide synthase-2 (NOS2), glucocorticoid receptor (NR3C1) and other targets, regulating platelet activation, peroxisome proliferator-activated receptor (PPAR), vascular endothelial growth factor A (VEGF) and other signaling pathways, and found key components: sennoside B, (+)-catechin, emodin, physcion, rhein, aloe-emodin, chrysophanol, gallic acid. HPLC was used to explore the dissolution rate of key components. The results showed that the contents of catechin, emodin, chrysophanol and physcion were significantly increased after RRR-PS combination (P < 0.01). In summary, RRR-PS has a significant effect on promoting blood circulation and removing blood stasis, and its mechanism of action is related to promoting angiogenesis, anti-coagulation, anti-thrombosis and anti-inflammation. The efficacy is related to the change of solvent system and the large dissolution of catechin, emodin, chrysophanol and physcion after the RRR-PS combination. The results of the study can further provide a reference for the follow-up study on the active substances and mechanism of the RRR-PS combination. Animal experiments have been approved by the Experimental Animal Committee of Shaanxi University of Traditional Chinese Medicine (No. SUCMDL20210309002).
