Exploring the risk "time interval window" of sequential medication of Reduning injection and penicillin G injection based on the correlation between biochemical indexes and metabolomics characteristics
10.16438/j.0513-4870.2023-1311
- VernacularTitle:基于生化指标与代谢组学特征关联探索热毒宁注射液与青霉素注射液序贯用药的风险“时间窗”
- Author:
Ming-liang ZHANG
1
;
Yu-long CHEN
2
;
Xiao-yan WANG
1
,
3
;
Xiao-fei CHEN
1
;
Hui ZHANG
1
;
Ya-li WU
1
;
Liu-qing YANG
1
;
Shu-qi ZHANG
1
;
Lu NIU
1
;
Ke-ran FENG
1
;
Wei-xia LI
1
,
3
;
Jin-fa TANG
1
,
3
,
4
Author Information
1. Department of Pharmacy, the First Affiliated Hospital of Henan University of Chinese Medicine, Henan Province Engineering Research Center for Clinical Application, Evaluation and Transformation of Traditional Chinese Medicine, Henan Provincial Key Laboratory for Clinical Pharmacy of Traditional Chinese Medicine, Henan Province Engineering Research Center of Safety Evaluation and Risk Management of Traditional Chinese Medicine, Zhengzhou 450003, China
2. Henan University of Chinese Medicine, Zhengzhou 450046, China
3. Provincial and Ministerial Co-construction Collaborative Innovation Center for Prevention and Treatment of Respiratory Diseases with Traditional Chinese Medicine of Henan University of Chinese Medicine, Zhengzhou 450046, China
4. Henan University of Chinese Medicine, Zhengzhou 450046, China
- Publication Type:Research Article
- Keywords:
combination of Chinese and Western medicine;
adverse drug reaction;
time interval window;
Reduning injection;
penicillin G injection;
pseudo-allergic reaction;
metabonomics;
arachidonic acid metabolism
- From:
Acta Pharmaceutica Sinica
2024;59(7):2098-2107
- CountryChina
- Language:Chinese
-
Abstract:
Exploring the risk "time interval window" of sequential medication of Reduning injection (RDN) and penicillin G injection (PG) by detecting the correlation between serum biochemical indexes and plasma metabonomic characteristics, in order to reduce the risk of adverse reactions caused by the combination of RDN and PG. All animal experiments and welfare are in accordance with the requirements of the First Affiliated Experimental Animal Ethics and Animal Welfare Committee of Henan University of Chinese Medicine (approval number: YFYDW2020002). The changes of biochemical indexes in serum of rats were detected by enzyme-linked immunosorbent assay. It was determined that RDN combined with PG could cause pseudo-allergic reactions (PARs) activated by complement pathway. Further investigation was carried out at different time intervals (1.5, 2, 3.5, 4, 6, and 8 h PG+RDN). It was found that sequential administration within 3.5 h could cause significant PARs. However, PARs were significantly reduced after administration interval of more than 4 h. LC-MS was used for plasma metabolomics analysis, and the levels of serum biochemical indicators and plasma metabolic profile characteristics were compared in parallel. 22 differential metabolites showed similar or opposite trends to biochemical indicators before and after 3.5 h. And enriched to 10 PARs-related pathways such as arachidonic acid metabolism, steroid hormone biosynthesis, linoleic acid metabolism, glycerophospholipid metabolism, and tryptophan metabolism. In conclusion, there is a risk "time interval window" phenomenon in the adverse drug reactions caused by the sequential use of RDN and PG, and the interval medication after the "time interval window" can significantly reduce the risk of adverse reactions.
