Identification of cajanonic acid A metabolites in rats by UPLC-Q-TOF-MS/MS
10.16438/j.0513-4870.2023-1451
- VernacularTitle:UPLC-Q-TOF-MS/MS法鉴定大鼠中树豆酮酸A代谢产物
- Author:
Yao CHENG
1
,
2
,
3
;
Yu-juan BAN
1
,
2
;
Rui CHEN
1
,
2
;
Li ZHANG
1
,
2
;
Ke-rong HU
1
,
2
,
3
;
Jing HUANG
4
Author Information
1. Guizhou Medical University, Guizhou Provincial Engineering Technology Research Center for Chemical Drug R &
2. D, Guiyang 550004, China
3. School of Pharmacy, Guizhou Medical University, Guiyang 550004, China
4. School of Pharmacy, Guizhou Medical University, Guiyang 550004, China
- Publication Type:Research Article
- Keywords:
cajanonic acid A;
UPLC-Q-TOF-MS/MS;
metabolite
- From:
Acta Pharmaceutica Sinica
2024;59(5):1382-1390
- CountryChina
- Language:Chinese
-
Abstract:
This research established a simple, rapid and sensitive ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) method to investigate the metabolic profiles of cajanonic acid A (CAA) in rats. After intragastric administration of CAA (30 mg·kg-1) to rats, the biological samples were detected by UPLC-Q-TOF-MS/MS. Relevant data was collected and processed, the accurate mass and MS2 spectra of the metabolites were compared with the parent compound. As a result, a total of 23 metabolites were detected, including 15 in urine, 11 in bile, 11 in feces, and 9 in plasma. The major metabolic pathways related to CAA included dehydrogenation, reduction, hydroxylation, methylation and glucuronide conjugation. This experiment was approved by Animal Ethics Committee of Guizhou Medical University (approval number: 1603137).
