Quality evaluation of Callicarpa nudiflora from Hainan Province based on simultaneous determination of six anti-inflammatory active components by HPLC
10.16438/j.0513-4870.2023-1382
- VernacularTitle:高效液相色谱法同时测定海南裸花紫珠中6种抗炎活性成分的含量与质量评价
- Author:
Juan CHEN
1
,
2
,
3
;
Hong HU
1
,
2
,
3
;
Yue SHI
1
,
2
,
3
;
Xing-dong KANG
4
;
Shu-mei WANG
1
,
2
,
3
;
Yuan-yuan XIE
1
,
2
,
3
Author Information
1. School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China
2. Guangdong Provincial Traditional Chinese Medicine Quality Engineering and Technology Research Center, Guangzhou 510006, China
3. Key Laboratory of Digital Quality Evaluation Technology of Traditional Chinese Medicine, National Administration of Traditional Chinese Medicine, Guangzhou 510006, China
4. Jiangxi Puzheng Pharmaceutical Co., Ltd., Ji’an 331409, China
- Publication Type:Research Article
- Keywords:
italic>Callicarpa nudiflora Hook. et Arn.;
anti-inflammation;
network pharmacology;
Q-marker;
multi-index quantitative analysis;
quality evaluation
- From:
Acta Pharmaceutica Sinica
2024;59(5):1408-1421
- CountryChina
- Language:Chinese
-
Abstract:
The anti-inflammatory efficacy of Callicarpa nudiflora extract were evaluated upon lipopolysaccharide (LPS)-induced infective inflammation in rats. Pathological changes of lung and colon tissues observed with hematoxylin-eosin (H&E) staining, together with inflammatory factors in serum, including nitric oxide (NO), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), were applied to assess the mitigating effects of C. nudiflora water extract on model rats. The experimental protocol strictly adhered to the guidelines of the Ethics Committee of Animal Research of Guangdong Pharmaceutical University (Approval: gdpulac2022132). Quality markers with anti-inflammatory activities were recognized by network pharmacology analysis. Subsequently, a reliable method for simultaneously quantifying six components was established. As a result, the pathological injury on lung and colon tissues of model rats induced by LPS were improved by C. nudiflora water extract. Compared with model group, C. nudiflora extract had decreased the release of proinflammatory factors in serum, including TNF-α and IL-6, and reduced the NO (P < 0.01). Network pharmacological analysis obtained 83 chemical components, 466 component targets, 584 disease targets and 129 action targets, which were mainly concentrated in 624 biological processes such as inflammatory response and positive regulation of nitric oxide biosynthesis, as well as 162 pathways such as phosphatidylinositol-3-hydroxykinase-protein kinase (PI3K-Akt) signal pathway and Janus kinase-signal transducer and activator of transcription 3 (JAK-STAT3) signal pathway. Upon analyzing their specificity, effectiveness, detectability, and quantity transfer rule from herb to extract, 6-hydroxyluteolin 7-glucoside, cynaroside, caffeic acid, acteoside, isoacteoside, and forsythoaside B were identified as the quality markers. Their anti-inflammatory activities were verified with LPS-induced inflammation model of RAW264.7 cells. Then contents of these 6 quality markers in 16 batches of C. nudiflora were determined. Thereby, the anti-inflammatory efficacy of C. nudiflora extract were confirmed in vivo, 6-hydroxyluteolin 7-glucoside, cynaroside, caffeic acid, acteoside, isoacteoside, and forsythoaside B were identified as anti-inflammatory ingredients, which can be used as quality control indicators for the herb and related formulation.
