Investigation of metabolites of 2-methyl-dechloroketamine in vitro
10.16438/j.0513-4870.2023-1191
- VernacularTitle:苯环己哌啶类物质甲胺酮体外代谢研究
- Author:
Jun-hui RU
1
;
Feng HUANG
1
;
Hao WU
2
;
Min-hao WANG
1
;
Xing KE
1
;
Jian-hong GAO
3
;
Yi-lei FAN
1
Author Information
1. Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province, Zhejiang Police College, Hangzhou 310053, China
2. Dian Regional Forenic Science Institute·Zhejiang, Hangzhou 310007, China
3. Key Laboratory of Drug Monitoring and Control of Zhejiang Province, National Anti-drug Laboratory Zhejiang Regional Center, Hangzhou 310053, China
- Publication Type:Research Article
- Keywords:
2-methyl-deschloroketamine;
liver microsome;
hair;
metabolite;
metabolic pathway
- From:
Acta Pharmaceutica Sinica
2024;59(5):1357-1363
- CountryChina
- Language:Chinese
-
Abstract:
The abuse of novel phenylcyclohexylpyridine drugs poses a significant threat to societal safety. The novel psychoactive substance 2-methyl-deschloroketamine (2-MDCK), belonging to the phenylcyclohexylpyridine class, has recently surfaced as a new compound. However, there is a lack of understanding regarding its metabolic pathways and the identification of suitable biomarkers. In this study, a human liver microsomal model was established, and ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technology was applied to investigate the in vitro metabolism and products of 2-MDCK. The results indicate that 2-MDCK undergoes various metabolic reactions, including dehydrogenation, deamination, hydroxylation, and demethylation, leading to the formation of eight metabolites. By conducting actual testing on hair samples from 2-MDCK abusers, the existence of six metabolites was confirmed. Comparing the metabolic products in the liver microsomal in vitro model, M3.1 and M4.1 were identified as biomarkers for 2-MDCK consumption. Five samples of abusers of 2-methyl-dechloroketamine were provided by the Anti-Drug Brigade of the Hangzhou Public Security Bureau. Negative hair samples were provided by laboratory volunteers, and all samples were obtained with the informed consent of the volunteers. These findings provide a scientific basis for the detection and identification of 2-MDCK and its metabolites, as well as crucial support for the study of the metabolic mechanisms of similar novel psychoactive substances in the phenylcyclohexylpyridine class. This research holds significant importance in addressing the issue of abuse of new psychoactive substances.
