The Effect and Mechanism of Estrogen and Estrogen Receptors in the Pathogenesis of Non-Invasive Fungal Rhinosinusitis
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20241021.007
- VernacularTitle:雌激素及其受体在非侵袭性真菌性鼻-鼻窦炎发病过程中的作用及其机制
- Author:
Chuanmei YANG
1
;
Yafei SU
;
Luhua SHEN
;
Hong ZHANG
;
Teng WANG
;
Siyu JI
;
Zhiyong XIAO
Author Information
1. 福州市第二总医院耳鼻咽喉科,福建福州 350007
- Keywords:
non-invasive fungal rhinosinusitis;
estrogen receptor;
eosinophils;
mast cells;
NF-κB;
IKK;
MASPIN
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2024;45(6):1017-1025
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore the role of estrogen and estrogen receptor (ER) in the pathogenesis and regulation of non-invasive fungal rhinosinusitis (NIFR).[Methods]Totally 60 patients with NIFS who met the inclusion criteria in Fuzhou Second General Hospital from November 2020 to November 2023 were selected as the NIFS group,while 30 healthy volunteers were recruited as the blank control group. Samples of each group were collected. The number of eosinophils and mast cells in each group were detected by HE staining;ER expression was detected by immunohistochemistry and immunofluorescence;mRNA expression levels of NF-κB,IKK and MASPIN were detected by qPCR;and protein expression levels of NF-κB,IKK and MASPIN were detected by immunohistochemistry and Western blot.[Results]In the NIFS group,the counts of eosinophils and mast cells were significantly increased respectively,compared with those in the control group,and the inter-group differences are statistically significant (P<0.01 and P<0.05,respectively). The Estrogen Receptor (ER) score in the NIFS group was significantly increased compared with that in the control group,and the inter-group differences are statistically significant (P<0.05). Additionally,the average high-density value in the NIFS group was significantly increased compared with that in the control group,and the inter-group differences are statistically significant (P<0.01). The expression levels of NF-κB,IKK,and MASPIN in the NIFS group were significantly increased respectively,compared with those in the control group,and the inter-group differences are statistically significant (P<0.01,P<0.05,and P<0.01,respectively). The mRNA expression levels of NF-κB,IKK,and MASPIN in the NIFS group were significantly increased,respectively,compared with those in the control group (P<0.01). Furthermore,the protein expression levels of NF-κB,IKK,and MASPIN in the NIFS group were increased,respectively,and the inter-group differences are statistically significant (P<0.01,P<0.01,and P<0.05,respectively).[Conclusion]Our results show that the significant increase in the number of eosinophils and mast cells,and in the expression levels of ER,NF-κB,IKK and MASPIN may indicate a significant increase in eosinophil and mast cell infiltration in ER positive patients,and suggest the involvement of estrogen and its receptors in the pathogenesis of NIFS.
