Impact and mechanism of curcumin on endometriosis model rats
- VernacularTitle:姜黄素对子宫内膜异位症模型大鼠的影响及机制
- Author:
Cuimei QIN
1
;
Xinyi LIU
1
;
Xiaolan DING
1
;
Huiling GUO
2
;
Pinying CHEN
2
;
Jiao LIU
3
;
Jie CHENG
2
Author Information
1. Dept. of Gynecology，Binzhou Hospital of Traditional Chinese Medicine，Shandong Binzhou 256601，China
2. Dept. of Pharmacy，Hebei Provincial Hospital of Traditional Chinese Medicine，Shijiazhuang 050011，China
3. Dept. of Chinese Medicine Pharmacology，Hebei University of Chinese Medicine，Shijiazhuang 050200，China
- Publication Type:Journal Article
- Keywords:
endometriosis;
curcumin;
Notch1 signaling
- From:
China Pharmacy
2024;35(22):2744-2749
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the impact and mechanism of curcumin on endometriosis （EMS） model rats based on Notch1 signaling pathway. METHODS Female SD rats with synchronized estrous cycles were implanted with autologous endometrium on the abdominal wall to construct EMS model. EMS rats were randomly divided into model group， low-， medium- and high-dose groups of curcumin （60， 120， 240 mg/kg）， and Notch 1 inhibitor DAPT group （7 mg/kg）. The sham surgery group was also established， with 10 rats in each group. Rats in each group received intragastric administration or injection via caudal veins with the corresponding drugs for 4 weeks. Endometriotic lesions were observed and measured using ultrasound and visual inspection， and their volumes were calculated. Histopathological morphology of the lesion tissues was observed. The levels of interleukin-1β （IL-1β）， IL-6， and tumor necrosis factor-α （TNF-α） in the abdominal cavity fluid， as well as the mRNA and protein expression levels of Notch1， matrix metalloproteinase-9 （MMP-9）， and vascular endothelial growth factor （VEGF） in the endometriotic lesions were measured. RESULTS Compared with the model group， the volume of endometriotic lesions in the low- ， medium- and high-dose groups of curcumin and the DAPT group was significantly decreased （P＜0.05）； atrophy or disappearance of columnar epithelium， cyst disappearance； the levels of IL-1β， IL-6， and TNF- α （except for the low-dose curcumin group） in the abdominal cavity fluid， as well as the mRNA （except for MMP-9 and VEGF in the low-dose curcumin group） and protein （except for MMP-9 in the low-dose curcumin group） expression levels of Notch1， MMP-9， and VEGF in the endometriotic lesions were significantly decreased （P＜0.05）. The curcumin high-dose group and DAPT group showed superior results in most indicators compared to the curcumin low- and medium-dose groups. CONCLUSIONS Curcumin has an improving effect on EMS， and its mechanism may be related to inhibiting Notch1 signaling pathway， reducting local inflammatory responses， and inhibiting ectopic endometrial invasion and angiogenesis.
