Quality evaluation of Lycopodium japonicum from different producing areas
- VernacularTitle:不同产地伸筋草的质量评价
- Author:
Rui ZHANG
1
,
2
,
3
;
Xiaochuan FENG
1
,
2
,
3
;
Yanzhao XU
4
;
Baohai XU
1
,
2
Author Information
1. Dept. of Chinese Pharmacy，Beijing Jishuitan Hospital，Capital Medical University，Beijing 100035，China
2. The Fourth Clinical Medical College of Peking University，Beijing 100035，China
3. National Center for Orthopedics，Beijing 100035，China
4. National Institutes for Food and Drug Control，Beijing 100050，China
- Publication Type:Journal Article
- Keywords:
Lycopodium japonicum;
HPLC;
chemometric
- From:
China Pharmacy
2024;35(22):2732-2738
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To evaluate the quality of Lycopodium japonicum from different producing areas. METHODS Sixteen batches of L. japonicum from six provinces were used as samples. The contents of α -obscurine， β -obscurine， lycopodine， lycodoline， lyclaninol， 21-episerratenediol， stigmasterol and β-sitosterol in L. japonicum were detected by quantitative analysis of multi-components by single-marker （QAMS）. The results were compared with the results of external standard method. The extract and total ash were measured according to the appendix of Chinese Pharmacopoeia. Principal component analysis， orthogonal partial least squares-discriminant analysis and weighted technique for order preference by similarity to ideal solution （TOPSIS） methods were combined to evaluate its quality. RESULTS The contents of lyclaninol in the 16 batches of L. japonicum were 0.183-0.446 mg/g. Using lyclaninol as internal reference material， the contents of α-obscurine， β-obscurine， lycopodine， lycodoline， 21-episerratenediol， stigmasterol and β-sitosterol were 1.250-2.097， 0.690-1.184， 0.035-0.102， 0.076-0.150， 0.356-0.570， 0.085-0.229 and 0.238-0.855 mg/g， respectively， with no significant difference from the results of external standard method. The extract was 12.18%-22.78%， and the total ash was 3.16%-6.11%. Principal component analysis showed that samples S1-S6，S7-S11，S12-S16 could be clustered into 3 categories. The variable importance in projection values for α-obscurine， β-sitosterol， lyclaninol， β-obscurine and 21- episerratenediol were all greater than 1. The weighted TOPSIS evaluation results showed that sample S14 had the best quality （Jb＝ 0.709 2）. CONCLUSIONS L. japonicum from Suijiang county of Yunnan province had the best quality. The established QAMS combined with chemometric and weighted TOPSIS methods can be used for quality evaluation of L. japonicum from different producing areas.
