The material basis and mechanism of action of anti-inflammatory effects of simplified Zhiqin Decoction
10.16438/j.0513-4870.2023-0817
- VernacularTitle:简化栀芩汤的抗炎药效物质基础及作用机制研究
- Author:
Kun WANG
1
,
2
;
Yang LIU
1
,
2
;
Yue YIN
1
,
2
;
Xiao XIAO
1
,
2
;
Xue-jiao ZHOU
1
,
2
;
Zhi-ying YUAN
1
,
2
;
Liang-hong YE
3
;
Xiao-yu XU
1
,
2
,
4
,
5
,
6
Author Information
1. School of Pharmacy &
2. School of Traditional Chinese Medicine, Southwest University, Chongqing 400715, China
3. Tongliang District Hospital of Traditional Chinese Medicine in Chongqing, Chongqing 402560, China
4. Southwest University Hospital, Chongqing 400715, China
5. State Administration of Traditional Chinese Medicine High-level Chinese Medicine Pharmacology Key Construction Discipline, Chongqing 400715, China
6. Chongqing Chinese Medicine Rehabilitation Key Construction Discipline, Chongqing 400715, China
- Publication Type:Research Article
- Keywords:
simplified Zhiqin Decoction;
inflammatory pharmacodynamics;
mechanism of action;
pharmacodynamic material basis;
network pharmacology
- From:
Acta Pharmaceutica Sinica
2024;59(8):2245-2254
- CountryChina
- Language:Chinese
-
Abstract:
The anti-inflammatory effect of simplified Zhiqin Decoction was observed by using lipopolysaccharide (LPS)-induced inflammation mouse model. The main chemical constituents and the main mechanism of action of simplified Zhiqin Decoction were predicted by network pharmacology. Animal experiments verified the anti-inflammatory mechanism of simplified Zhiqin Decoction (this experiment was approved by the Animal Experiment Ethics Committee of Southwest University, approval number: IACUC-20210825-02). Simplifying Zhiqin Decoction has a significant anti-inflammatory effect on inflammatory mice, can significantly improve the overall macro shape of mice, reduce body temperature, water intake, increase the number of autonomous activities; alleviate liver, lung, spleen, thymus inflammation and pathological damage; decrease tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6, nitric oxide (NO), prostaglandin E2 (PGE2) content in serum and urine. The contents of serum immune factors IgG, IgA and IgM were increased. Network pharmacology predicted 66 potential anti-inflammatory active components of simplified Zhiqin Decoction involving 132 inflammatory targets, and the key anti-inflammatory signaling pathway involved PI3K/AKT, TNF, JAK-STAT, etc. Animal experiments show that simplified Zhiqin Decoction can significantly reduce the expression of JAK2/STAT-PI3K/AKT-NF-κB-TNF signaling pathway related proteins in lung tissue of inflammatory mice. The results of this study showed that simplified Zhiqin Decoction had significant anti-inflammatory effects, and its main anti-inflammatory components were quercetin, β-sitosterol, kaempferol, wogonin, stigmasterol, luteolin, neobaicalein, etc. The main mechanism of its anti-inflammatory action is that it significantly inhibits the expression of JAK2/STAT-PI3K/AKT-NF-κB-TNF signaling pathway protein.
