Identification and expression analysis of AP2/ERF gene family in Artemisia argyi
10.16438/j.0513-4870.2024-0277
- VernacularTitle:艾AP2/ERF基因家族鉴定及表达模式分析
- Author:
Xue-xue YUE
1
;
Chuang XIAO
1
;
Qian-wen ZHANG
1
;
Sai-nan PENG
1
;
Chang-jie CHEN
1
;
Jia ZHOU
1
;
Jin-xin LI
1
;
Yu-kun LI
1
;
Yu-huan MIAO
1
,
2
;
Da-hui LIU
1
,
2
Author Information
1. School of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China
2. Hubei Shizhen Laboratory, Wuhan 430065, China
- Publication Type:Research Article
- Keywords:
italic>Artemisia argyi;
active component;
italic>AP2/ERF transcription factor;
bioinformatics;
expression pattern
- From:
Acta Pharmaceutica Sinica
2024;59(9):2634-2647
- CountryChina
- Language:Chinese
-
Abstract:
italic>Artemisia argyi is a traditional Chinese medicine in China, which is used as medicine with its leaves. The leaves of A. argyi mainly contain flavonoids, phenolic acids, volatile oils and other compounds, and have a variety of pharmacological activities. AP2/ERF transcription factors are abundant in plants and are mainly involved in plant growth and development, abiotic stress response and secondary metabolite biosynthesis regulation. However, there are few reports on the AP2/ERF gene family and its functions in A. argyi. In this study, we systematically identified the AP2/ERF gene family in A. argyi genome, and analyzed its phylogenetic tree, protein physicochemical properties, subcellular localization, conserved motifs, promoter elements, and expression patterns. The results showed that a total of 204 AP2/ERF transcription factors were identified in A. argyi genome, encoding proteins consisting of 88-483 amino acids with a relative molecular mass of 10-52.94 kDa and a theoretical isoelectric point of 4.62-9.88. Subcellular prediction showed that the majority of AP2/ERFs were located in the nucleus, cytoplasm, and the minority of them is located in the membrane and chloroplasts. According to the Arabidopsis AP2/ERF family classification, A. argyi AP2/ERF proteins were divided into four subfamilies: Soloist, AP2, ERF (B3, B4, B5, B6), and DREB (A1, A2, A4, A5, A6), among which DREB family accounted for the largest proportion, and the same subfamily had similar conserved motifs. cis-Acting element analysis showed that AP2/ERF promoters have a large number of elements responding to light and abiotic stress. Expression pattern analysis showed that most of the genes of the AP2/ERF family were dominantly expressed mainly in roots and stems, of which 19 were dominantly expressed in leaves, 77 would be induced to be expressed by methyl jasmonate, of which 16 genes were both dominantly expressed in leaves and induced to be expressed by methyl jasmonate, and these AP2/ERF genes may be the key regulatory genes for the synthesis of active ingredients in A. argyi leaves.This study lays the foundation for the functional study of AP2/ERF family genes and their regulating roles in the active components biosynthesis in A. argyi leaves.
