Two new isocoumarins from cave-derived Metarhizium anisopliae NHC-M3-2
10.16438/j.0513-4870.2024-0211
- VernacularTitle:溶洞真菌Metarhizium anisopliae NHC-M3-2中两个新的异香豆素类化合物
- Author:
Rong-xiang WU
1
;
Jie-lan GUO
1
;
Hua HUANG
1
;
Jing-jing LIAO
1
;
Yi HAO
1
;
Fan-dong KONG
1
;
Li-man ZHOU
1
;
Chao-jun ZHANG
2
Author Information
1. Key Laboratory of Universities in Guangxi for Excavation and Development of Ancient Ethnomedicinal Recipes, Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, School of Chemistry and Chemical Engineering, Guangxi Minzu University, Nanning 530006, China
2. Zhejiang Kanglaite Pharmaceutical Limited Company, Hangzhou 310018, China
- Publication Type:Research Article
- Keywords:
italic>Metarhizium anisopliae NHC-M3-2;
tumor cytotoxicity;
isocoumarin compound;
secondary metabolite
- From:
Acta Pharmaceutica Sinica
2024;59(9):2588-2593
- CountryChina
- Language:Chinese
-
Abstract:
Extracting extracts of secondary metabolites from the karst cave fungus Metarhizium anisopliae NHC-M3-2 from the Yilingyan Scenic Area in Guangxi. Ten compounds were isolated and purified from fungal secondary metabolites using thin-layer chromatography and high-performance liquid chromatography. 7-Hydroxy-3-hydroxypropyl-5,6-dimethylisochrome-1-one (1) and 7-hydroxy-3,5,6-trimethyl-isochromen-1-one (2) were new isocoumarin compounds, N-acetyl phenylalanine (3), chaetosumin J (4), 2-one-13-hydroxy-3,5,8,7(11)-eudesmatetraen-12,8-olide (5), 1H-indole-3-carboxaldehyde (6), irpexolaceus B (7), irlactin L (8), cytochalasin K (9), and helvolic acid (10), a total of 8 known compounds. The structure of the compound was determined using methods such as NMR and mass spectrometry. The tumor cytotoxicity of the compound was evaluated using the CCK-8 method. The results showed that the IC50 of compound 2 on HepG2 cells was 29.83 µmol·L-1, and compound 9 on HCT116 cells was 27.44 µmol·L-1.
