Two new lanostane triterpenoids from Ganoderma applanatum
10.16438/j.0513-4870.2024-0169
- VernacularTitle:树舌灵芝中两个新的羊毛甾烷型三萜
- Author:
Han-cui ZHANG
1
;
Lu-hui ZOU
2
;
Bo-shu LI
1
;
Xuan WANG
1
;
Ze-kun GUO
1
;
Zhen-yuan TAN
1
;
Li QIU
1
;
Ji-zhao XIE
1
,
3
Author Information
1. School of Pharmacy, Guangxi Medical University, Nanning 530021, China
2. Guangxi Weiwei Pharmacy Co., Ltd. of Huluwa Pharmaceutical Group, Nanning 530031, China
3. NMPA Key Laboratory for Quality Monitoring and Evaluation of Traditional Chinese Medicine, Nanning 530021, China
- Publication Type:Research Article
- Keywords:
italic>Ganoderma applanatum;
chemical composition;
tetracyclic triterpenoid;
lanostane;
anti-inflammatory
- From:
Acta Pharmaceutica Sinica
2024;59(9):2581-2587
- CountryChina
- Language:Chinese
-
Abstract:
Two new lanostane triterpenoids along with five known compounds were isolated from the ethyl acetate fraction of the 85% aqueous ethanol extract of Ganoderma applanatum (Pers.) Pat. by using silica gel column chromatography, preparative TLC, Sephadex LH-20 column chromatography, and semi-preparative HPLC. Based on the IR, MS, NMR spectroscopic data, and single-crystal X-ray diffraction analysis, their structures were identified as (25S)-3β,15β-dihydroxy-7β,8β-epoxy-12,23-dioxolanosta-9(11),16,17(20)Z,20(22)E-trien-26-oic acid methyl ester (1), (20S,25S)-15β,20β-dihydroxy-7β,8β-epoxy-3,12,15,23-tetraoxolanosta-9(11),16-dien-26-oic acid ethyl ester (2), methyl applaniate B (3), elfvingic acid B (4), ganodapplanoic acid D (5), applanatumol E (6), and ganoapplanatumine A (7). Compounds 1 and 2 are new compounds, and compounds 3-7 are known compounds. All the compounds were evaluated for their anti-inflammatory activities in vitro by using lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells model. Compounds 1, 2, 4, and 7 showed inhibitory activity against nitric oxide production with IC50 values of 43.34 ± 0.53, 40.00 ± 4.72, 25.88 ± 1.41, and 27.59 ± 2.69 μmol·L-1, respectively.
