Exploration of the antioxidant role and mechanism of Astragalus membranaceus based on a glucose-induced Caenorhabditis elegans model
10.16438/j.0513-4870.2023-1423
- VernacularTitle:基于葡萄糖诱导的秀丽隐杆线虫模型探讨黄芪抗氧化的作用及机制
- Author:
Mei-mei YANG
1
;
Han-ying LIU
1
;
Mei-zhong PENG
1
;
Pan MA
1
;
Yi-ting NIU
1
;
Teng-yue HU
1
;
Yu-xing JI
1
;
Gai-mei HAO
2
;
Jing HAN
3
Author Information
1. Beijing Key Laboratory of TCM Syndrome and Formula, Key Laboratory of TCM Syndrome and Formula of the Ministry of Education, School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China
2. Institute of Basic Theory for Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China
3. Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China
- Publication Type:Research Article
- Keywords:
italic>Astragalus membranaceus;
italic>Caenorhabditis elegans;
oxidative stress;
protein skinhead-1;
high glucose
- From:
Acta Pharmaceutica Sinica
2024;59(9):2556-2563
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study was to observe the effect of Astragalus membranaceus on high sugar-induced Caenorhabditis elegans, and to explore its mechanism of action. UPLC-MS method was used to identify the components of Astragalus membranaceus. A high glucose model was established by using Caenorhabditis elegans as a model organism, and the effects of Astragalus membranaceus on body length, body bending, swallowing frequency, and reactive oxygen species (ROS) of the nematode were determined; the effects of Astragalus membranaceus on the expression of mRNA of genes related to the protein skinhead-1 (SKN-1) signaling pathway were examined by using the real-time fluorescence quantitative polymerase chain reaction (PCR). The results showed that compared with the normal group, the nematode body length, body bending, and swallowing frequency expression were significantly reduced and the ROS content in the body was significantly increased in the high glucose state; after the administration of Astragalus membranaceus, the body length, body bending, and swallowing frequency expression were significantly increased, and the ROS content was significantly reduced (P < 0.01). Compared with the normal group, SKN-1, superoxide dismutas-3 (SOD-3), glutathione S-transferase 4 (GST-4), and glutathione S-transferase 7 (GST-7) expression were significantly decreased in Caenorhabditis elegans in the high glucose condition; SKN-1, SOD-3, GST-4, and GST-7 expression were significantly increased after administration of Astragalus membranaceus (P < 0.01). In the present study, we demonstrated that Astragalus membranaceus has an effect on high glucose-induced Caenorhabditis elegans nematodes, and its mechanism of action may be through the modulation of the SKN-1 signaling pathwaym in order to ameliorate the oxidative stress response induced by high glucose.
