Development and application of a method for identifying Pheretima and a common counterfeit of Metaphire magna based on signature peptides
10.16438/j.0513-4870.2024-0316
- VernacularTitle:基于特征多肽的地龙鉴别与伪品保宁腔蚓检查方法的建立及应用
- Author:
Rui LIU
1
,
2
;
Jing-xian ZHANG
3
;
Qing HU
3
;
Jian SUN
3
;
Hong YU
3
;
Ying-ying RAN
3
;
Fan HUANG
3
;
Xiu-hong MAO
3
;
Shen JI
1
,
2
Author Information
1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2. Shanghai Institute for Food and Drug Control, Shanghai 201203, China
3. Shanghai Institute for Food and Drug Control, Shanghai 201203, China
- Publication Type:Research Article
- Keywords:
italic>Pheretima;
italic>Metaphire magna;
signature peptide;
quality standard;
Xiaohuoluo pill;
Shenjindan capsule
- From:
Acta Pharmaceutica Sinica
2024;59(10):2842-2848
- CountryChina
- Language:Chinese
-
Abstract:
Based on the species-specific peptides of Pheretima and its common counterfeit (Metaphire magna), an identification method was established using ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry (UHPLC-MS/MS) for quality evaluation of Pheretima and its preparations. Separation was performed on a CORTECS T3 C18 column with 0.1% formic acid and acetonitrile as the mobile phases. Mass spectrometry with multiple reaction monitoring (MRM) using ESI+ mode was used to simultaneously monitor three ion pairs. The results indicated that the method was specific and could distinguish Guang Dilong, Hu Dilong, and M. magna, which were consistent with those of DNA barcode identification. The adulteration test showed that the LOD of peptide M was 1 μg·g-1. Peptide M could be detected when 1% M. magna was added to Guang Dilong, indicating the high sensitivity of the method. Fifty-four batches of commercially available samples contained 35% Guang Dilong, 35% Hu Dilong, and 15% M. magna. No ions were detected in 15% of the samples, and DNA barcode identification revealed that they were mainly from Amynthas, with similar appearance to Hu Dilong. The analysis results of the formulation showed that no peptide ions were detected in 3 batches of Xiaohuoluo pills (3/6) and M. magna were detected in 2 batches of Shenjindan capsules (2/4). The developed method in the study has good specificity, sensitivity, and feasibility, and could be used for quality control of Pheretima and its related preparations. It is of great significance for improving quality standards and regulating the medicinal market of Pheretima.
