Identification and expression pattern analysis of FBXL gene family in Salvia miltiorrhiza
10.12206/j.issn.2097-2024.202407034
- VernacularTitle:丹参FBXL基因家族的鉴定和表达模式分析
- Author:
Ruiyang YAO
1
;
Haizheng YU
;
Yaoxin LI
;
Lei ZHANG
Author Information
1. 海军军医大学药学系,上海 200433
- Keywords:
Salvia miltiorrhiza;
F-box-LRR gene family;
Bioinformatics;
Functional analysis
- From:
Journal of Pharmaceutical Practice and Service
2024;42(11):461-470
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify and analyze the bioinformatics and expression patterns of the F-box-LRR(FBXL)gene family of Salvia miltiorrhiza based on genomic data,and provide a foundation for further elucidating its gene functions.Methods The SmFBXL gene was identified from the Salvia miltiorrhiza genomic database.Its gene structure features,promoter cis-acting elements,physicochemical properties of encoded proteins,evolutionary relationships,and tissue expression were analyzed by bioinformatics methods and online tools.Results A total of 104 SmFBXL genes were identified from the Salvia miltiorrhiza genome,unevenly distributed on 8 chromosomes,with upstream promoters containing cis-acting elements related to plant stress resistance,growth and development,and hormone response.A phylogenetic tree of the FBXL family members of Salvia miltiorrhiza,Arabidopsis thaliana,and Glycine max was constructed,dividing the 104 SmFBXL genes into 7 subfamilies.Through homologous evolution analysis,it was speculated that SmFBXL36 might be involved in defense against pathogen invasion,SmFBXL86 and SmFBXL79 might play important roles in regulating lateral root growth in Salvia miltiorrhiza,and SmFBXL11 and SmFBXL40 might regulate hypocotyl growth.Transcriptome data showed differential expression of SmFBXL genes in different tissues of Salvia miltiorrhiza,with 13 SmFBXL genes showing higher expression levels in roots and leaves,serving as candidate genes for further research on the SmFBXL gene family.Conclusion The research results provided a reference for further elucidating the regulatory mechanisms of SmFBXL genes in stress response and secondary metabolite biosynthesis in Salvia miltiorrhiza.
