Identification of in Vitro and in vivo Chemical Constituents of Ruyi Zhenbaowan Based on UHPLC-Q Exactive Orbitrap HRMS
10.13422/j.cnki.syfjx.20241443
- VernacularTitle:基于UHPLC-Q Exactive Orbitrap HRMS的如意珍宝丸体内外成分分析
- Author:
Kedian CHEN
1
;
Zhaochen MA
1
;
Bingbing CAI
1
;
Ying LIU
1
;
Yudong LIU
1
;
Tao LI
1
;
Mingzhu XU
1
;
Haiping WANG
2
;
Na LIN
1
;
Yanqiong ZHANG
1
Author Information
1. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China
2. Arura Tibetan Medicine Co. Ltd.， Xining 810003， China
- Publication Type:Journal Article
- Keywords:
Ruyi Zhenbaowan;
UHPLC-Q Exactive Orbitrap HRMS;
chemical constituent;
constituent absorbed into plasma;
constituent absorbed into cerebrospinal fluid
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(24):78-84
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo identify the chemical constituents of Ruyi Zhenbaowan in vitro and in vivo. MethodThe chemical constituents of Ruyi Zhenbaowan were identified based on UHPLC-Q Exactive Orbitrap HRMS. A total of 12 male SD rats were randomized into two groups： control （pure water） and Ruyi Zhenbaowan （1.8 g·kg-1）. The rats were administrated with the suspension of Ruyi Zhenbaowan or pure water by gavage. After 1.5 h， the plasma and cerebrospinal fluid were collected. Chromatographic separation was performed on a Waters ACQUITY UPLC BEH C18 column （2.1 mm × 150 mm， 1.7 μm） with a mixture of 0.1% formic acid aqueous solution （A） and acetonitrile （B） as the mobile phase. Gradient elution was carried out according to the procedure of 0~15 min，97%~80%A；15~30 min ，80%~60%A；30~40 min，60%~30%A；40~45 min，30%~5%A. The ion source was electrospray ionization， and scan range was m/z 100-1 500. The prototype components and the components in the plasma and cerebrospinal fluid were analyzed qualitatively by scanning in positive and negative ion modes and identified by comparison with the data in published literature and the information of standard substances. ResultA total of 126 chemical constituents were identified from the 80% methanol solution of Ruyi Zhenbaowan， and 14 and 7 prototype constituents were detected in the plasma and the cerebrospinal fluid， respectively. In addition， the fragmentation rules of apigenin， apigenin-7-O-glucuronide， galangin， liquiritin， piperine， glycyrrhizic acid， eugenol， gallic acid， and cholic acid were deduced. ConclusionThis study achieved rapid multicomponent characterization and identification of Ruyi Zhenbaowan in vitro and in vivo， providing theoretical support for exploring active substances and performing quality control.l.
