Analysis of inorganic elements in different batches of earthworm polypeptides by ICP-MS combined with chemometrics technology
10.16438/j.0513-4870.2023-1187
- VernacularTitle:ICP-MS法结合化学计量学分析不同批次地龙多肽中无机元素的含量
- Author:
Hong-liu YANG
1
;
Wei-ting ZHONG
2
;
Yu-shi GUO
3
;
Shu-qi LI
1
;
Jin-chai QI
1
;
Yong-gang LIU
1
;
Tao MA
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102401, China
2. Beijing Tide Pharmaceutical Co., Ltd., Beijing 100176, China
3. Baotou Kundulun District Hospital, Baotou 014010, China
- Publication Type:Research Article
- Keywords:
ICP-MS;
microwave digestion;
earthworm;
earthworm polypeptide;
inorganic element
- From:
Acta Pharmaceutica Sinica
2024;59(4):1040-1047
- CountryChina
- Language:Chinese
-
Abstract:
To establish a method for determining 26 inorganic elements in earthworm polypeptide and determine the elemental content in different batches of earthworm polypeptide, microwave digestion method was used to pre-treat the samples, and ICP-MS method was used to determine the content of 26 elements in different batches of earthworm polypeptide. The linear relationships of 26 elements were good in the range of 0-1 000 μg·L-1, with R2 greater than 0.999, precision RSD 0.21%-2.71%, repeatability RSD 0.19%-4.69%, stability RSD 0.11%-4.24%, and recovery rates of 82.41%-116.16%. The data was plotted using Origin 2022 software to characterize the distribution of elements content. SPSS 27.0 was used for principal component analysis, and SIMCA 14.1 software was used for OPLS-DA analysis. The results showed that among the 26 elements, the higher content of earthworm polypeptide was K, Na, and Ca, followed by Zn, Fe, Al, B and other trace elements, In, Sc, Co, Pb, Bi and other elements had little or no detectable content, and there were differences in the content of polypeptide in different batches. This study provides a theoretical basis for the production quality control, quality evaluation and drug efficacy application of earthworm polypeptide through the determination and analysis of the elements and content of earthworm polypeptide.
