Enhancing production of emestrin in Emericella sp. 1454 by adding the biosynthetic precursor glutathione
10.16438/j.0513-4870.2023-0894
- VernacularTitle:谷胱甘肽促进Emericella sp. 1454中emestrin的产量提高
- Author:
Yu-chuan CHEN
;
Tong-mei XIAO
;
Bing-jie SU
;
Bi-ying YAN
;
Li-yan YU
;
Shu-yi SI
;
Ming-hua CHEN
- Publication Type:Research Article
- Keywords:
biosynthetic precursor;
glutathione;
emestrin;
yield improvement;
fermentation medium optimization;
italic>Emericella sp.;
epipolythiodioxopiperazine
- From:
Acta Pharmaceutica Sinica
2024;59(4):1087-1091
- CountryChina
- Language:Chinese
-
Abstract:
Based on the genomic information of Emericella sp. 1454, in conjunction with literature analysis of its secondary metabolite emestrin, this study identified the biosynthetic precursors of emestrin and enhanced its production by supplementing the culture medium with these precursors. In this study, it was found for the first time that the addition of biosynthetic precursor, reduced glutathione, to the culture medium significantly increased emestrin yield. By incorporating 1.5 g of reduced glutathione into 50 g of rice culture medium and fermenting for 15 days, a yield of 30.82 mg of emestrin was obtained, which marked an 11.71-fold increase compared to the original fermentation approach. The method is both simple and cost-effective, establishing a solid foundation for the efficient synthesis of emestrin and similar compounds. Additionally, it serves as an important reference for enhancing the production of other epipolythiodioxopiperazine compounds.
