Research progress on protein engineering technology and its application in the synthesis biology of medicinal natural products
10.16438/j.0513-4870.2024-0105
- VernacularTitle:蛋白质工程技术及其在药用天然产物合成生物学中的应用研究进展
- Author:
Xiao-yan SUN
;
Jing-jing CHEN
;
Tian-jiao CHEN
;
Ting GONG
;
Jin-ling YANG
;
Ping ZHU
- Publication Type:Research Article
- Keywords:
medicinal natural product;
biosynthesis;
enzyme;
protein engineering;
synthetic biology
- From:
Acta Pharmaceutica Sinica
2024;59(6):1601-1615
- CountryChina
- Language:Chinese
-
Abstract:
Natural products are important sources of drug discovery. However, the traditional methods of extraction and isolation, as well as chemical synthesis for obtaining natural products are associated with issues such as operational complexity, high costs, low efficiency, and environmental pollution. Constructing microbial cell factories through synthetic biology methods to produce medicinal natural products has the advantages of high efficiency, low cost, and environmental protection. Nevertheless, the scope and yield improvement of the products are limited by the limitations of enzymes in microbial cell factories. Protein engineering is considered one of the most effective approaches to overcome these limitations. This article introduces commonly used methods of protein engineering technology and summarizes its specific applications in improving enzyme performance, modifying the enzymatic environment, and promoting the development of synthetic biology tools in the field of pharmaceutical natural product synthesis. Furthermore, it analyzes the current bottlenecks and challenges in protein engineering and looks forward to its future application prospects, offering insights for the development and practical use of protein engineering technology.
