Sappanone A attenuates renal ischemia-reperfusion injury in rats by regulating JNK signal pathway
10.16438/j.0513-4870.2023-1356
- VernacularTitle:苏木酮A调控JNK通路减轻大鼠肾脏缺血再灌注损伤
- Author:
Tai-wei JIN
;
Xiao-ning GAO
;
Wen-lin SONG
;
Yan-yan WANG
;
Lin SUN
;
Ling-hong LU
- Publication Type:Research Article
- Keywords:
sappanone A;
renal ischemia-reperfusion injury;
p-JNK/JNK;
p-ERK/ERK;
anisomycin
- From:
Acta Pharmaceutica Sinica
2024;59(6):1639-1646
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to investigate the role and mechanism of sappanone A (SA) in regulating renal ischemia-reperfusion injury (IRI) in rats. The animal experiment has been approved by the Ethics Committee of Suzhou Wujiang District Children's Hospital (approval number: 2022010). First, hematoxylin-eosin (H&E) staining was used to evaluate the effects of SA on IRI, and renal damage was scored. Serum creatinine (SCr), blood urea nitrogen (BUN) and cystatin C (Cystatin C) were analyzed. The effect of sappanone A on the apoptosis of renal tubular epithelial cells induced by IRI was analyzed by TUNEL staining. Protein expression levels of p-JNK/JNK, p-ERK/ERK, Bcl2, Bax and cleaved-caspase 3 in renal tissues were detected by Western blot. Finally, H&E staining, serological analysis, TUNEL staining and Western blot were used to determine whether JNK activator anisomycin could reverse the effect of SA on IRI in rats. The results showed SA significantly reduced the renal tubule injury caused by ischemia-reperfusion, and decreased the level of SCr, BUN and Cys C in serum. TUNEL staining showed that SA significantly reduced the apoptosis of renal tubular epithelial cells induced by IRI. Western blot analysis of kidney tissue showed that SA significantly promoted the expression of apoptosis inhibiting protein Bcl2 and inhibited the expression of apoptosis-promoting proteins Bax and cleaved-caspase 3. Further analysis elucidated that SA did not affect the phosphorylation of ERK but decreased the phosphorylation of JNK. Finally, H&E staining, serological analysis, TUNEL staining and Western blot confirmed that JNK activator anisomycin could reverse the alleviating effect of SA on IRI in rats. The above findings suggest that SA could alleviate IRI in rats by inhibiting JNK phosphorylation.
