Establishment and evaluation methods of a novel animal model of liver depression transforming into fire syndrome-related depression
10.16438/j.0513-4870.2023-1118
- VernacularTitle:一种新型肝郁化火型抑郁症动物模型的建立及评价方法
- Author:
Dan SU
1
;
Jian LI
1
;
Gen-hua ZHU
1
;
Ming YANG
1
,
2
;
Liang-liang LIAO
1
;
Zhi-fu AI
1
;
Hui-zhen LI
1
,
3
;
Ya-li LIU
1
,
4
;
Yong-gui SONG
1
,
3
Author Information
1. Key Research Laboratory for Chinese Medicine Efficacy Evaluation (Prevention and Treatment of Mental Disorders Brain Disease) of Jiangxi Traditional Chinese Medicine Administration/Key Research Laboratory for Depression Syndrome Animal Models of Jiangxi Traditional Chinese Medicine Administration, Jiangxi University of Chinese Medicine, Nanchang 330004, China
2. Jiangxi Guxiang Jinyun Comprehensive Health Industry Co., Ltd., Nanchang 330029, China
3. Key Laboratory of Pathological Research on Experimental Animals, Nanchang, Jiangxi University of Chinese Medicine, Nanchang 330006, China
4. Key Laboratory of Pharmacodynamics and Safety Evaluation, Health Commission of Jiangxi Province, Nanchang Medicine College, Nanchang 330004, China
- Publication Type:Research Article
- Keywords:
epression;
animal model;
liver depression transforming into fire;
Danzhi Xiaoyao powder;
combination of disease and syndrome
- From:
Acta Pharmaceutica Sinica
2024;59(6):1680-1690
- CountryChina
- Language:Chinese
-
Abstract:
Through a compound induction method, combined with neurobehavioral, macroscopic characterization and objective pathological evaluation indicators, a murine depression model of liver depression transforming into fire syndrome was constructed and confirmed. The model was constructed using a combination of sleep deprivation, light exposure, and alternate-day food deprivation. Evaluation was conducted at three levels: face validity, constructs validity, and predictive validity. The establishment of the liver depression transforming into fire syndrome depression model was further validated through the counterproof of traditional Chinese medicine formulas. In terms of face validity, compared to the control group, mice in the model group exhibited typical depressive symptoms in neurobehavioral assessments; the general observation of the model group mice reveals disheveled and lackluster fur, along with delayed and easily agitated responses. Additionally, there is a substantial increase in water consumption. In the sleep phase detection of mouse, the model group showed a significant increase in the proportion of time spent in the wake phase during sleep, accompanied by a significant decrease in the proportions of time spent in both non-rapid eye movement (NREM) and rapid eye movement (REM) sleep phases. There are significant differences in physiological indicators such as average blood flow velocity, blood flow rate, tongue, urine, and claw color (r values) in the internal carotid artery. Structural validity demonstrated that levels of 5-hydroxytryptamine (5-HT), dopamine (DA), and γ-aminobutyric acid (GABA) in the hippocampus of model mice decreased significantly, while acetylcholine (ACh), tryptophan (Try), and glutamic acid (Glu) levels increased significantly. Treatment with Danzhi Xiaoyao San led to varying degrees of restoration in the aforementioned neurotransmitters. In terms of predictive validity, the antidepressant paroxetine effectively ameliorated depressive behaviors in the model mice, and the classic formula Danzhi Xiaoyao San demonstrated varying degrees of improvement in depression-related indicators. In conclusion, this study has established a novel animal model of liver-stagnation with the fire depression, thereby expanding the repertoire of traditional Chinese medicine depression models. This development contributes to the diversification of melancholic syndrome models in Chinese medicine, offering a broader spectrum of options for scientific exploration, efficacy evaluation, and drug screening in the future prevention and treatment of depression with traditional Chinese medicine. Animal experiments were conducted with the approval and supervision of the Animal Ethics Committee of Jiangxi University of Traditional Chinese Medicine (ethics number: 20230313040).
