Effect of hydrogen sulfide on TGF⁃ β1mediated epithelial⁃mesench ymal transformation in bleomycin⁃induced pulmonary fibrosis rats
https://kns.cnki.net/kcms2/article/abstract?v=YMwpULBJqz7IPKcB63hN57v9ZHwtOMkIXz0-S29l8Qy5omStTi5Q1lvtktvFNbkndF5DBF1adORCKAMQz0P32SNZYFi_kq3-qEMMM6Hv5h_Swax2whm_S9Sz7gBolQyLx0TF6JagULrt_rSKtAOIxYBxKQvr2uFyHqOoXw9-YcprVMB05Sk6mcg-gB42LE3_&uniplatform=NZKPT&language=CHS
- Author:
Rongfang Tu
1
;
Saili Zeng
1
;
Zhenhua He
1
;
Xiaowu Tan
1
;
Zhe Chen
1
;
Yezhou Xia
1
;
Xuehua Li
1
Author Information
1. Dept ofPulmonary and Critical Care Medicine , The Second Afiliated Hospital ,Dept ofPulmonary and Critical Care Medicine ,Hengyang Medical School , University of South China ,Hengyang 421001
- Publication Type:Journal Article
- Keywords:
hydrogen sulfide;
pulmonary fibrosis;
epithelial⁃mesenchymal transition;
TGF⁃ β1
- From:
Acta Universitatis Medicinalis Anhui
2022;57(1):77-83
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To observe the effect of hydrogen sulfide( H2 S) on the expression of transforming growth factor⁃β1(TGF⁃ β1) , E ⁃cadherin (E⁃CAD) , Vimentin (VIM) , alpha⁃smooth muscle actin ( α ⁃SMA) during epithelial⁃mesenchymal transformation(EMT) , and to explore the anti⁃fibrosis mechanism of it.
Methods : Sixty rats ( male SD) were randomly divided into control group, bleomycin group, NaHS + bleomycin group and prednisolone + bleomycin group , 15 rats per group. 5 rats of each group were sacrificed at random in 7th , 14th and 28th day. The degree of alveolitis and pulmonary fibrosis was observed . The expression of protein and mRNA of TGF⁃ β1 , E ⁃cad , VIM , α ⁃SMA were determined by Immunohistochemi stry and RT⁃PCR.
Results :① HE and Masson staining showed that the lung tissue of fibrosis had the lowest degree in control group. and the most severe in bleomycin group. The lung tissue of NaHS + bleomycin group and prednisolone + bleomycin group also had alveolitis and fibrosis changes , but the degree Were significantly less than bleomycin group. ② The mRNA and protein expression levels of TGF⁃ β1 , VIM and α ⁃SMA in bleomycin group, NaHS + bleomycin group and prednisolone + bleomycin group were all higher than that in control group at 7th , 14th and 28th day ( P < 0. 05 ) , while the expression levels of them in NaHS + bleomycin group and prednisolone + bleomycin group were all lower than that in bleomycin groupat each time (P < 0. 05) , which was significant at 28th day in NaHS + bleomycin. ③ The mRNA and protein expression levels of E ⁃Cad in bleomycin group, NaHS + bleomycin group and prednisolone + bleomycin group were all lower than that in control group at 7th , 14th and 28th day(P < 0. 05) , but the expression levels of E ⁃Cad in⁃NaHS + bleomycin group and prednisolone + bleomycin group were higher than that in bleomycin group at each time(P < 0. 05) , which was significant at 28th day in NaHS + bleomycin.
Conclusion :H2 S can reduce the degree of pulmonary fibrosis in rats , its mechanism may be related to the down⁃regulation of TGF⁃ β1and the inhibition of the EMT , which can enhance the expression of E ⁃cad and reduce the expression of TGF⁃ β1 , VIM and α ⁃SMA.
- Full text:2024112916413047757硫化氢对博来霉素致肺纤维化...介导上皮间充质转化过程影响_涂容芳.pdf
