Inhibitory effect of transglutaminase 2 gene knockdown on proliferation and invasion of lung cancer cells
10.19405/j.cnki.issn1000-1492.2022.01.012
- Author:
Jianxin Zhang
1
;
Yulu Liu
2
;
Zigui Zhu
2
;
Hong Zhao
3
;
Pingbo Yao
4
Author Information
1. Dept of Thoracic Surgery , Nanhua Hospital Afiliated to Nanhua University , Hengyang 421002
2. Dept of Critical Care Medicine , Nanhua Hospital Afiliated to Nanhua University , Hengyang 421002
3. Dept of Basic Nursing , School of Nursing , Nanhua University , Hengyang 421001
4. Changsha Vocational College of Civil Afairs , Changsha 410004
- Publication Type:Journal Article
- Keywords:
transglutaminase 2;
lung cancer;
proliferation;
invasion
- From:
Acta Universitatis Medicinalis Anhui
2022;57(1):59-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To analyze the effects of transglutaminase 2(TGM2) gene interference on the malignant proliferation and movement of lung cancer cells .
Methods : A549 cells in logarithmic growth phase were divided into control group , shRNA⁃NC group and TGM2⁃shRNA1 group . After transfection , expression levels of TGM2 mRNA A549 cells in logarithmic growth phase were divided into control group , shRNA⁃NC group and TGM2⁃shRNA1 group . After transfection , expression levels of TGM2 mRNA clone formation assay . The proliferation activity of cells was detected by CCK⁃8 . The apoptosis rate was detected by flow cytometer. The invasion of cells was detected by Transwell . The number of tubule formation in each group was observed under microscope . The expression of E ⁃cadherin (E⁃cad) , N ⁃cadherin (N⁃cad) , fibronectin (FN) and vascular endothelial growth factor (VEGF) in each group was detected by Western blot .
Results :Compared with shRNA⁃NC group , the mRNA level and protein expression of TGM2 in TGM2⁃shRNA1 group were decreased (P <0. 05) , and the rate of clone formation , proliferation activity , number of invasive cells and number of tubules formation were decreased ( P < 0. 05) . The apoptosis rate and the expression of E ⁃cad protein were increased ( P <0. 05) , while the expression of N ⁃cad , FN and VEGF protein were decreased (P < 0. 05) .
Conclusion : TGM2 gene silencing can inhibit the expression of VEGF protein , inhibit the malignant proliferation and invasion of A549 cells , and promote apoptosis .
- Full text:2024112910174584627转谷氨酰胺酶2基因敲低对肺癌细胞增殖和侵袭的抑制作用_张建新.pdf
