Over⁃expression of miR⁃370 regulates lung inj ury in asthmatic mice and its mechanism
10.19405/j.cnki.issn1000-1492.2022.01.011
- Author:
Yanhong Ren
1
,
2
;
Lingyun Zhang
2
,
3
;
Shaocong Zhao
1
,
2
;
Guangchao Zhang
1
,
2
;
Xiaomin Sun
1
,
2
Author Information
1. Dept of General Internal Medicine , Afiliated Children &prime
2. s Hospital of Zhengzhou University, Zhengzhou 450000
3. Dept of Emergency, Afiliated Children &prime
- Publication Type:Journal Article
- Keywords:
bronchial asthma;
miR⁃370;
immune injury;
lung injury
- From:
Acta Universitatis Medicinalis Anhui
2022;57(1):53-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect and mechanism of overexpression of miR⁃370 on immune injury in asthmatic mice induced by ovalbumin ( OVA) .
Methods :40 female BALB/c mice were divided into control group , model group (OVA) , negative control group (OVA + miR⁃NC) and miR⁃370 overexpression group ( OVA+ miR⁃370) . Except for the control group , the other groups were sensitized and stimulated to establish the asthma mouse model. After successful modeling , the OVA + miR⁃NC group and the OVA + miR⁃370 group were injected with miR⁃NC adenovirus and miR⁃370 overexpression adenovirus respectively by caudal vein. RT⁃PCR was commended to detect the expression level of miR⁃370 , the bronchoalveolar lavage fluid ( BALF) was collected , and Wright - Giemsa staining was used to determine the BALF number of leukocytes. Enzyme⁃linked immunosorbent assay (ELISA) was employed to detect the expression levels of immunoglobulin E (IgE) and immunomodulatory factor protein in BALF of asthmatic mice ; hematoxylin⁃eosin (HE) staining and terminal labeling method (TUNEL) were used to observe the degree of lung tissue pathological damage and cell apoptosis ; Western blot was used to detect the protein expression levels of Bax/Bcl⁃2 and cleaved cas3/cas3.
Results : Compared with the control group , mice in the OVA group expressed low miR⁃370 (P < 0. 05) , the number of leukagtes increased (P < 0. 05) , the mice in the OVA group expressed low miR⁃370 (P < 0. 05) , the number of leukagtes increased (P < 0. 05) , the ( IL⁃13) , interleukin⁃12 (IL⁃12) and interleukin 4 (IL⁃4) were up⁃regulated (P < 0. 05) , and lung tissue was severely damage(P < 0. 05) , the expression ratio of Bax/Bcl⁃2 and cleaved cas3/cas3 increased (P < 0. 05) ; Compared with the OVA group , there was no difference in the indicators of the OVA + miR⁃NC grouup. Over⁃rexpression of miR⁃370 up⁃regulated the expression of miR⁃370 ( P < 0. 05) , decreased the number of leukagtes( P < 0. 05 ) and the level of IgE (P < 0. 05) , down⁃regulated immunoregulatory factors INF⁃γ and IL⁃12 (P < 0. 05) , up⁃regulated ( IL⁃13) IL⁃13 and IL⁃4(P < 0. 05) , and improved lung tissue damage , decreased cell apoptosis , and reduced the expression ratio of Bax/Bcl⁃2 and cleaved cas3/cas3(P < 0. 05) .
Conclusion : Overexpression of miR⁃370 inhibits the production of BALF inflammatory cells in OVA⁃induced asthmatic mice and relieves lung injury in mice.
- Full text:2024112910051059746过表达miR-370调控哮喘小鼠肺损伤及其机制_任彦红.pdf
