A preliminary study on the differential expression of circRNA in peripheral blood of Uygur patients with T2DM in Xinjiang
10.19405/j.cnki.issn1000-1492.2022.01.009
- Author:
Mengwei Tong
1
;
Xiangyun Chang
1
Author Information
1. Dept of Endocrine and Metabolic Diseases , The First Afiliated Hospital , School of Medicine ,Shihezi University,Shihezi 832008
- Publication Type:Journal Article
- Keywords:
type 2 diabetes mellitus;
Uygur nationality;
circular RNAs;
high⁃throughput nucleotide sequencing;
molecular biology;
pathogenesis
- From:
Acta Universitatis Medicinalis Anhui
2022;57(1):41-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the significance of circular RNAs (circRNAs) underlining the molecular biological pathogenesis of Uyghur type 2 diabetes (T2DM) by observing the difference of circRNAs expression in the peripheral blood of Uyghur T2DM patients.
Methods :High⁃throughput nucleotide sequencing was utilized to screen out differentially expressed circRNAs from peripheral blood mononuclear cells of 5 Uyghurs volunteers with healthy adults and 5 Uyghurs with T2DM. To further verify the differentially expressed circRNAs , additional samples from 20 NC Uyghur volunteers and 22 T2DM Uyghur patients were analyzed by real⁃time fluorescent quantitative⁃PCR(RT⁃qPCR) .
Results :A total of 134 differentially expressed circRNAs were screened out by high⁃throughput nucleotide sequencing , of which 87 were up⁃regulated and 47 were down⁃regulated. hsa⁃circ⁃0139634 and has⁃circ⁃0005414 were obviously up⁃regulated and hsa⁃circ⁃0032491 and hsa⁃circ⁃0002922 were obviously down⁃regulated and they were selected for further verification by RT⁃qPCR of the additional samples. The study found that the differential expression of hsa⁃circ⁃0139634、hsa⁃circ⁃0032491、hsa⁃circ⁃0002922 in peripheral blood samples was statistically significant , which was consistent with the sequencing results. Among them , the area under the receiver operating characteristic(ROC) curve of hsa⁃circ⁃0139634 was 0. 935 [95% confidence interval(0. 884 - 1. 000) , P< 0. 001] , Youden index was 0. 867 , sensitivity was 1. 000 , specificity was 0. 867 and cutoff value was 0. 169;the area under the receiver operating characteristic(ROC) curve of has⁃circ⁃0005414 was 0. 575[95% confidence inter⁃
cutoff value was 5. 749 ; the area under the ROC curve of hsa circ 0032491 was 0. 316[95%
confidence interval(0. 086 - 0. 440) , P = 0. 017] , Youden index was 0. 056 , sensitivity was 1. 000 , specificity was 0. 056 and cutoff value was 0. 230 ; the area under the ROC curve of hsa⁃circ⁃0002922 was 0. 263[95% confidence interval(0. 078- 0. 428) , P = 0. 014] , Youden index was 0. 080 , sensitivity was 0. 955 , specificity was 0. 125 and cutoff value was 0. 011 .
Conclusion :Uyghur T2DM patients and NC populations have differentially expressed circRNAs profiles. Of note , hsa⁃circ⁃0139634 has potential value in the molecular biological pathogenesis of Uyghur T2DM.
- Full text:2024112817213726485新疆维吾尔族T2DM患者外...rcRNA差异表达初步研究_仝梦维.pdf
