Effects of 60Co-γ radiation on the structure and anti-inflammatory activity of nialamide
10.13491/j.issn.1004-714X.2024.05.001
- VernacularTitle:钴60γ射线辐射对烟肼酰胺的结构及抗炎活性的影响
- Author:
Peng YAN
1
;
Jing HOU
2
;
Ping LI
3
;
Ruirui CHEN
1
;
Yan LI
1
Author Information
1. Department of Cardiovascular Medicine, The Second Affiliated Hospital of Air Force Medical University, Xi'an 710000 China.
2. Department of Clinical Medicine, Xi'an Public Health Center, Xi'an 710000 China.
3. Department of Pharmacy, Xi'an Hospital of Traditional Chinese Medicine, Xi'an 710000 China.
- Publication Type:OriginalArticles
- Keywords:
Ionizing radiation;
Nialamide;
60Co-γ ray;
Anti-inflammation;
RAW 264.7 cell
- From:
Chinese Journal of Radiological Health
2024;33(5):491-498
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of cobalt-60 gamma-ray (60Co-γ) radiation on the structure of Nialamide, compare the anti-inflammatory activity of irradiation products, and explore the mechanism of action. Methods After 60Co-γ irradiation of nialamide at a dose of 50 kGy, five known compounds were obtained (2-6). The viability of RAW 264.7 (mouse mononuclear macrophage leukemia) cells treated with these compounds was determined by CCK-8 assay. The secretion of interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2) and the content of nitric oxide (NO) were measured using enzyme-linked immunosorbent assay and Griess method. The production of reactive oxygen species (ROS) was detected using DCFH-DA fluorescent probe. The expression levels of cell-induced nitric oxide synthase (iNOS), cyclooxygenase (COX-2), nuclear transcription factor-κB (NF-κB), and IκB were detected using Western blot. Results The products of nialamide after irradiation did not significantly affect RAW264.7 cell viability (P > 0.05) but showed a strong anti-inflammatory effect (P < 0.01). Compared with nialamide, compounds 2, 3, 4, 6 significantly reduced NO content in LPS-induced RAW 264.7 cells (P < 0.01), and compound 4 had the most significant effect. Moreover, compound 4 significantly reduced the content of IL-6, TNF-α, PGE2, and ROS (P < 0.05) as well as the expression of iNOS, COX-2, NF-κB, and IκB (P < 0.05) in LPS-induced RAW 264.7 cells. Conclusion The chemical structure of nialamide is changed after irradiation with 60Co-γ, and its product compound 4 shows strong anti-inflammatory activity, which may be related to inhibiting the activation of NF-κB signaling pathway and reducing the release of inflammatory factors. Radiation technology can provide new insights into the changes of molecular structures and physiological properties of natural products.
