Antimicrobial activity of Ardisia serrata (Cavs.) Pers. ethanolic and aqueous leaf extract on the growth and biofilm formation of selected bacterial isolates

Patrick Josemaria DR Altavas; Alfonso Rafael G Abaya; Remo Vittorio Thaddeus D Abella; Danna Lee A Acosta; Angelica C Aguilar; Camille Anne V Aguinaldo; Katrina Loise L Aguirre; Catherine Therese C Amante; Karen B Amora; Glen Aldrix R Anarna; Rafael T Andrada; Gere Ganixon T Ang; Jeram Caezar R Angobung; Angelo V Aquino II; Dennielle Ann P Arabis; Hannah Luisa G Awitan; Mary Faith D Baccay; Chryz Angelo Jonathan B Bagsic; Tomas V Baldosano Jr; Cecilia C Maramba-lazarte

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Antimicrobial activity of Ardisia serrata (Cavs.) Pers. ethanolic and aqueous leaf extract on the growth and biofilm formation of selected bacterial isolates

Author: Patrick Josemaria DR. Altavas ¹ ; Alfonso Rafael G. Abaya ¹ ; Remo Vittorio Thaddeus D. Abella ¹ ; Danna Lee A. Acosta ¹ ; Angelica C. Aguilar ¹ ; Camille Anne V. Aguinaldo ¹ ; Katrina Loise L. Aguirre ¹ ; Catherine Therese C. Amante ¹ ; Karen B. Amora ¹ ; Glen Aldrix R. Anarna ¹ ; Rafael T. Andrada ² ; Gere Ganixon T. Ang ¹ ; Jeram Caezar R. Angobung ¹ ; Angelo V. Aquino II ¹ ; Dennielle Ann P. Arabis ¹ ; Hannah Luisa G. Awitan ¹ ; Mary Faith D. Baccay ¹ ; Chryz Angelo Jonathan B. Bagsic ¹ ; Tomas V. Baldosano Jr. ¹ ; Cecilia C. Maramba-Lazarte ³
Author Information

1. College of Medicine, University of the Philippines Manila
2. College of Medicine, University of the Philippines ManilaCollege of Medicine, University of the Philippines Manila
3. Institute of Herbal Medicine, National Institutes of Health, University of the Philippines Manila
Publication Type:Journal Article
Keywords: Ardisia serrata; Ardisia pyrmidalis; Cavs; Methicillin-sensitive Staphylococcus aureus; ethanolic extract; aqueous extract; antimicrobial
MeSH: Methicillin-resistant Staphylococcus aureus; Escherichia coli; natural product; biological products
From: Acta Medica Philippina 2024;58(18):91-97
CountryPhilippines
Language:English
Abstract: Background:Ardisia serrata (Aunasin) is an endemic Philippine plant of the family Primulaceae, with several studiesshowing the genus Ardisia as having potential antibacterial, antiangiogenic, cytotoxic, and antipyretic properties.
Objective:This study aims to determine the antibacterial and antibiofilm-forming activity of Ardisia serrata ethanolic and aqueous extracts on Escherichia coli, Methicillin-Sensitive Staphylococcus aureus (MSSA), and Methicillin-Resistant Staphylococcus aureus (MRSA).
Methods:This is an experimental study testing the activity against bacterial strains of E. coli, MSSA, and MRSA using ethanolic and aqueous extracts of A. serrata leaves. Microtiter susceptibility and biofilm inhibition assays were done with two-fold dilutions of the extract against the selected strains using spectrophotometry with optical density (OD) at 600 nm and 595 nm, respectively, to quantify bacterial growth and biofilm inhibition. The bacterial susceptibility and biofilm inhibition activity was reported as percent inhibition (PI). Minimum inhibitory concentration (MIC), and minimum biofilm inhibition concentration (MBIC) values were obtained using logarithmic regression of the PI values.
Results:A. serrata ethanolic extracts showed weak growth inhibitory activity against MSSA and MRSA with minimum inhibitory concentration (MIC) values of 2.6192 and 3.2988 mg/mL, respectively, but no biofilm inhibition activity was noted, while the aqueous extracts exhibited negligible biofilm inhibition activity against MSSA and MRSA with minimum biofilm inhibition concentration (MBIC) values of 13.5972 and 8964.82 mg/mL, respectively, and with no growth inhibition activity. Both ethanolic and aqueous extracts showed no growth inhibition and biofilm inhibition activities against E. coli.
Conclusion:Staphylococcus aureus is susceptible to the bioactivity of the leaf extracts of A. serrata and has potential to be used as an antibacterial in the treatment of infectious diseases.
Full text:202411080941246774412.pdf