Pharmacodynamic Effect and Mechanism of Xiaoke Drink in Ameliorating Insulin Resistance in ob/ob Mice
10.13422/j.cnki.syfjx.20242321
- VernacularTitle:消渴饮改善ob/ob小鼠胰岛素抵抗的药效学作用及其机制
- Author:
Baoying LI
1
;
Baosheng ZHAO
2
;
Yuling ZHA
1
;
Mi DENG
1
;
Luna NIU
1
;
Xuefei LI
1
;
Ruowei ZHU
1
;
Yu DONG
3
;
Lu JING
1
Author Information
1. Eye Hospital， China Academy of Chinese Medical Sciences，Beijing 100040，China
2. Beijing Research Institute of Chinese Medicine， Beijing University of Chinese Medicine， Beijing 100029，China
3. Guang'anmen Hospital， China Academy of Chinese Medical Sciences，Beijing 100053，China
- Publication Type:Journal Article
- Keywords:
Xiaoke drink;
ob/ob mice;
insulin resistance;
cholesterol;
mechanism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(23):254-260
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of Xiaoke drink on insulin resistance in ob/ob mice and explore the mechanism. MethodEighteen ob/ob mice were randomly assigned into model， Xiaoke drink （17.68 g·kg-1）， and atorvastatin （0.01 g·kg-1） groups （n=6）， and six C57BL/6 mice were selected as the normal group. Mice in the normal and model groups were administrated with the same amount of distilled water. Fasting body weight， weekly food intake， and weekly water intake were measured at a fixed time. Fasting plasma glucose （FPG） and 2-hour post-load plasma glucose （2 hPG） were measured before and after 8-week intervention. After intervention， total cholesterol （TC）， triglyceride （TG）， fasting insulin （FINS）， Homeostasis Model Assessment-Insulin Resistance （HOMA-IR）， blood routine， and alkaline phosphatase （ALP） were measured. Western blot was employed to determine the expression levels of ubiquitin-specific protease 20 （USP20） and 3-hydroxy-3-methylglutaryl coenzyme A reductase （HMGCR） in the liver. The pancreas was stained with hematoxylin-eosin for observation. ResultCompared with the model group， the Xiaoke drink group showed decreased body weight of ob/ob mice （P<0.05， P<0.01）， declined growth trend of body weight （P<0.05， P<0.01）， reduced weekly average water intake， lowered levels of FPG， 2 hPG， TC， and HOMA-IR （P<0.05， P<0.01）， and down-regulated expression level of USP20 in the liver （P<0.05）. HMGCR content was positively correlated with USP20 expression. In addition， Xiaoke drink promoted the recovery of islet tissue morphology and function in ob/ob mice. ConclusionXiaoke drink can ameliorate insulin resistance in ob/ob mice by inhibiting USP20/HMGCR expression， reversing cholesterol biosynthesis process， and reducing cholesterol level.
