Construction and Experimental Validation of Chemically-induced Cough Model Based on Cough Waveforms
10.13422/j.cnki.syfjx.20241409
- VernacularTitle:基于咳嗽波形构建化学刺激诱导的咳嗽模型及实验验证
- Author:
Yunhang GAO
1
;
Ling JIA
1
;
Han LI
1
;
Ling SONG
1
;
Cong GUO
1
;
Tengfei CHEN
1
;
Hongping HOU
1
;
Weiya CHEN
1
;
Guangping ZHANG
1
Author Information
1. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences， Beijing 100700，China
- Publication Type:Journal Article
- Keywords:
guinea pig model of cough;
whole-body plethysmography （WBP）;
capsaicin;
GK-A;
antitussive effect
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(23):230-236
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo construct a cough model induced by chemical stimuli by whole-body plethysmography （WBP） for counting coughs based on cough waveforms， and use this model to explore the antitussive effect of GK-A. MethodDifferent chemical stimuli were used to induce coughs in mice or guinea pigs. Respiratory waveforms were monitored by WBP， and the recognizable and typical cough waveforms were selected for cough counting. Guinea pigs were induced to cough with different concentrations of citric acid or capsaicin， and cough waveforms were used to optimize the stimulation conditions. The optimized guinea pig model of cough was validated with dextromethorphan， and the optimized guinea pig model of capsaicin-induced cough was used to evaluate the antitussive effect of GK-A. ResultWBP could count the coughs induced by capsaicin and citric acid in guinea pigs by recognizable and typical respiratory waveforms. The optimized stimulation conditions were capsaicin concentration of 100 µmol·L-1 and nebulization for 2 min. The validation results showed that compared with the model group， the dextromethorphan group of guinea pigs had reduced coughs （P<0.05） and prolonged cough latency （P<0.01）. GK-A prolonged the cough latency （P<0.05） and reduced coughs （P<0.05） in the mouse model of ammonia-induced cough. In the guinea pig model of capsaicin-induced cough， GK-A prolonged cough latency （P<0.05）， reduced coughs （P<0.05）， and decreased substance P （SP） content in the guinea pig serum （P<0.05， P<0.01）. ConclusionA guinea pig model of capsaicin-induced cough was successfully established based on cough waveform counting， which provided an objective and accurate cough counting method. GK-A has antitussive effects， possibly by inhibiting the neuropeptide SP.
