Regulatory Effect of Naoxintong Capsules on Short-chain Fatty Acids in Mice with Cerebral Ischemia-reperfusion Injury Based on LC-MS/MS
10.13422/j.cnki.syfjx.20241507
- VernacularTitle:基于LC-MS/MS探讨脑心通胶囊对脑缺血再灌注损伤小鼠短链脂肪酸的调控作用
- Author:
Jing ZHANG
1
;
Yu LI
1
;
Yuxin LEI
1
;
Mengli CHANG
1
;
Yanan WANG
1
;
Jing XU
1
;
Shihuan TANG
1
Author Information
1. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China
- Publication Type:Journal Article
- Keywords:
cerebral ischemia-reperfusion injury;
intestinal barrier;
Naoxintong capsules;
short-chain fatty acids;
LC-MS/MS
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(23):141-148
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the mechanism of Naoxintong capsules' intervention in cerebral ischemia-reperfusion by building a mouse cerebral ischemia-reperfusion model based on short-chain fatty acids. MethodC57BL/6J male mice were randomly divided into the sham group， model group， Naoxintong group （158.9 mg∙kg-1）， and Ginaton group （12.1 mg∙kg-1） according to the random number table method. The model of cerebral ischemia-reperfusion （MCAO/R） was prepared via the filament occlusion method. The effect of Naoxintong capsules on brain injury in MCAO/R mice was evaluated by the neuroethological score， cerebral infarction area determination， Nissl staining， and immunofluorescence staining. Hematoxylin-eosin （HE） staining and Western blot were employed to evaluate the effect of Naoxintong capsules on the intestinal barrier in MCAO/R mice. The content of short-chain fatty acids in mouse feces was detected by LC-MS/MS. ResultCompared to the sham group， the model group exhibited significant increases in the cerebral infarction area， neuroethological score， and cell apoptosis rate （P<0.01）， with a notable decrease in the number of Nissl bodies （P<0.01）. The protein expression levels of Claudin-1 and Occludin were significantly reduced （P<0.05）. Compared with the model group， the intervention of Naoxintong capsules significantly decreased the cerebral infarction area （P<0.05） and improved the neuroethological score （P<0.01） and cell apoptosis rate （P<0.01）， with the number of Nissl bodies （P<0.01） and expression levels of Claudin-1 and Occludin proteins （P<0.01） increased. LC-MS/MS results showed that compared to the sham group， the model group featured a significantly reduced content of acetic acid， propionic acid， and butyric acid in feces （P<0.01）， while valeric acid， isovaleric acid， and isobutyric acid levels were increased （P<0.01）. The intervention of Naoxintong capsules notably lowered the content of valeric acid， isovaleric acid， and isobutyric acid （P<0.01）. ConclusionNaoxintong capsules can improve brain and intestinal barrier damage and play a protective role in cerebral ischemia-reperfusion by regulating the content of short-chain fatty acids.
