Effect of Changju Zhidong Prescription on Intestinal Flora in Children with Tic Disorder
10.13422/j.cnki.syfjx.20250566
- VernacularTitle:菖菊止动方对抽动障碍患儿肠道菌群的影响
- Author:
Qiang ZHANG
1
;
Tuo LIU
2
;
Junhong WANG
1
;
Yurou YAN
1
;
Ting SUN
1
;
Chengcheng LIU
2
Author Information
1. Dongzhimen Hospital，Bejjing University of Chinese Medicine，Beijing 100700，China
2. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
- Publication Type:Journal Article
- Keywords:
tic disordor;
intestinal flora;
Changju Zhidong prescription;
pediatrics;
traditional Chinese medicine;
clinical pharmacology
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(23):104-110
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the regulatory effect of Changju Zhidong prescription on gut microbiota structure of children with tic disorder（TD）. MethodTwenty-four children with TD who visited the pediatric outpatient clinic of Dongzhimen Hospital of Bejjing University of Chinese Medicine from November 2020 to January 2022 were selected as the observation group， and eight healthy children were selected as the normal group. The observation group was treated with Changju Zhidong prescription for 12 weeks， and the clinical efficacy was observed by Yale global tic severity scale（YGTSS）， traditional Chinese medicine（TCM） syndrome score， and score of TCM symptom evaluation scale for pediatric gastrointestinal tract. Meanwhile， The fecal samples of the observation and normal groups were collected before and after treatment， and 16S rDNA sequencing was used to explore the effects of Changju Zhidong prescription on the distribution of gut microbiota of the children. ResultCompared with the pre-treatment， the YGTSS， TCM syndrome score， and score of TCM symptom evaluation scale for pediatric gastrointestinal tract of the observation group were significantly reduced after 12 weeks of treatment（P<0.05）， Simpson and Pieloue indices in alpha diversity were significantly reduced（P<0.05）. Before treatment， the relative abundance of Streptococcus parasanguini in the observation group was significantly higher than that of the normal group， while the relative abundances of Citrobacter， Lactobacillus， Lacticaseibacillus， Muribaculaceae unclassified， Odoribacter and 7 other bacterial groups were significantly lower than that in the normal group. Compared with the pre-treatment， the observation group showed a significant decrease in Ruminococcus torques group unclassified and Ruminococcus torques group， and a significant increase in Olsenella， Acetivibrio and 4 other bacterial groups. Functional enrichment analysis showed that the main pathways of Changju Zhidong prescription regulating gut microbiota were bacterial secretion system， insect hormone biosynthesis， limonene and pinene degradation， novobiocin biosynthesis， tropane piperidine and pyridine alkaloid biosynthesis. ConclusionThe pathogenesis of children with TD is accompanied by intestinal flora disorders， and Changju Zhidong prescription can improve core and gastrointestinal symptoms， increase the abundance of beneficial and decrease the abundance of harmful gut microbiota in children with TD， and its mechanism may be through the metabolism of terpenoids and polyketides.
