Effect of Thyme Herbal Tea on Proliferation of Human Coronavirus OC43 in vitro and in vivo
10.13422/j.cnki.syfjx.20241504
- VernacularTitle:百里香药茶对人冠状病毒OC43体内外增殖的影响
- Author:
Jixiang TIAN
1
;
Tongtong ZHANG
2
;
Yuning CHANG
2
;
Peifang XIE
2
;
Shuwei DONG
2
;
Xiaoang ZHAO
3
;
Yun WANG
3
;
Chunhui ZHAO
3
;
Hongwei WU
3
;
Amei ZHANG
2
;
Haizhou LI
2
;
Xueshan XIA
4
;
Huamin ZHANG
1
Author Information
1. Institute of Basic Theory for Chinese Medicine， China Academy of Chinese Medical Sciences， Beijing 100700， China
2. Faculty of Life Science and Technology， Kunming University of Science and Technology， Kunming 650500， China
3. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs， Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China
4. Yunnan Provincial Key Laboratory of Public Health and Biosafety， Kunming Medical University， Kunming 650500， China
- Publication Type:Journal Article
- Keywords:
thyme herbal tea;
human coronavirus OC43 （HCoV-OC43）;
antiviral activity;
cell model;
mouse model
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(23):81-89
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of thyme herbal tea （BLX） on the proliferation of human coronavirus OC43 （HCoV-OC43） in vitro and in vivo. MethodThe chemical composition of BLX was analyzed by UPLC-MS. The cytotoxicity of BLX in HRT-18 cells and the effect of BLX treatment on the proliferation of HCoV-OC43 in cells were analyzed. Copies of viral gene were detected by real-time PCR. The effect of BLX treatment on the life cycle of HCoV-OC43 was detected by time-of-addition assay. The maximum tolerated dose of BLX and the influences of BLX on the body weight and survival time of suckling mice infected with HCoV-OC43 were determined. The expression of viral protein in the brain and lung tissue was analyzed by immunohistochemistry. ResultThere were 11 chemical components identified in BLX by UPLC-MS. BLX showed the 50% cytotoxic concentration （CC50） of （13 859.56±319） mg·L-1， the median inhibitory concentration （IC50） of （1 439.09±200） mg·L-1， and the selection index of 8.26-11.44 for HCoV-OC43 in HRT-18 cells. Compared with the cells infected with HCoV-OC43， BLX at the concentrations of 1 500， 1 000， 500 mg·L-1 inhibited the proliferation of this virus （P<0.05， P<0.01）. BLX exhibited antiviral effect in the early stage of virus infection， and the inhibition role in the attachment stage was more significant than that in the entry stage （P<0.05）. In the suckling mice infected with HCoV-OC43， BLX at 1200 and 600 mg·kg-1·d-1 alleviated the symptoms， prolonged the survival period， reduced the death rate， and down-regulated the mRNA level of nucleocapsid protein in the mice. Moreover， BLX at 1 200 mg·kg-1·d-1 down-regulated the expression of nucleocapsid protein in the brain （P<0.01） and the lung （P<0.01）. ConclusionBLX contained multiple antiviral ingredients. It inhibited the proliferation of HCoV-OC43 both in vitro and in vivo by interference with viral attachment. This study provides theoretical reference for the treatment of acute respiratory tract infection with HCoV-OC43 and for further development and application of BLX.
