Analysis of resistance situation and resistance genes of clinical isolates of carbapenem-resistant Klebsiella pneumoniae and Escherichia coli
10.12206/j.issn.2097-2024.202309059
- VernacularTitle:耐碳青霉烯类肺炎克雷伯菌及大肠埃希菌临床分离株耐药性及耐药基因分析
- Author:
Yun HUANG
1
;
Zhengyin ZHANG
1
;
Ying JIN
1
;
YiJing ZHENG
1
;
Tiejun LI
1
;
Lili SUN
1
Author Information
1. a. Department of Clinical Laboratory, b. Department of Pharmacy, Punan Branch of Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200125, China.
- Publication Type:Originalarticles
- Keywords:
carbapenem-resistant enterobacteriaceae;
phenotype of carbapenemase;
carbapenemase resistance gene;
resistance
- From:
Journal of Pharmaceutical Practice and Service
2024;42(10):439-444
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the current status of anti-bacterial activity of carbapenem-resistant Klebsiella pneumoniae and Escherichia coli clinically isolated from hospitalized patients, detect their related resistance genes, and provide reference for the clinical treatment of carbapenem resistant Enterobacteria （CRE） infections and the rational use of antibiotics. Methods A total of 400 non-repetitive isolates of Klebsiella pneumoniae and Escherichia coli isolated from clinical specimens of Punan Branch of Renji Hospital, Shanghai Jiao Tong University School of Medicine from January 2022 to December were collected. The minimum inhibitory concentrations of these strains against commonly used antibiotics were determined by the broth microdilution method. The carbapenemase and related resistance genes of CRE were detected by drug resistance phenotype testing and PCR. Results Among the 400 strains, 51 strains were identified as CRE, accounting for 12.75%. Among these, 49 strains produced carbapenemases, with 41 strains （80.39%） being CR Klebsiella pneumoniae and 10 strains （19.61%） being CR Escherichia coli. Among the CRE strains, 34 strains （66.67%） carried blaKPC, 13 strains （25.49%） carried blaNDM, and 2 strains （3.92%） carried blaOXA-48. Conclusion Compared with other commonly used antibiotics, colistin and tigecycline exhibited good in vitro antibacterial activity against carbapenemase-producing Klebsiella pneumoniae and Escherichia coli. In addition, there was good concordance between drug resistance phenotype testing and genotyping. Clinical microbiology laboratories could continuously monitor the drug resistance phenotype and genotype of CRE and develop appropriate treatment plans based on actual conditions.
