The effect of miR⁃9 on the proliferation of neural stem cells by regulating β⁃tubulin Ⅲ and GFAP
10.19405/j.cnki.issn1000-1492.2022.01.003
- Author:
Yu Wang
1
;
Lan Zhao
1
;
Bohong Kan
1
;
Huiyan Shi
1
Author Information
1. Dept ofAcupuncture , The First Afiliated Hospital of Tianjin University of Traditional Chinese Medicine , Tianjin 300193
- Publication Type:Journal Article
- Keywords:
neural stem cells;
neurodegenerative diseases;
glial cells;
differentiation
- From:
Acta Universitatis Medicinalis Anhui
2022;57(1):10-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore whether miR⁃9 affects the proliferation of neural stem cells (NSCs) by regulating β ⁃tubulin Ⅲ and glial fibrillary acidic protein (GFAP) .
Methods : NSCs cells were isolated and cultured on the purchased healthy pregnant mice and the isolated cells were divided into NO group ( non⁃transfected NSCs cell line) , NN group (NSCs transfected with miR⁃9 ⁃NC) , NM group (NSCs transfected) transfected with miR⁃9 mimics) , NI group ( transfected with miR⁃9 inhibitor) . Nestin was identified by immunofluorescence , the contents of Mir⁃9 , β ⁃tubulin Ⅲ and GFAP were measured by QRT⁃PCR , the proliferation of NSCs was measured by MTT method , the apoptosis of NSCs was measured by flow cytometry , and the protein expressions of Mir⁃9 , β ⁃tubulin Ⅲ and GFAP were detected by Western blot.
Results :The detection index Nestin of NSCs for 24 h after cell passage was positive in 90% of the NSCs , and co⁃localized with the nucleus , indicating the successful isolation of NSCs. The expression of miR⁃9 mRNA in NSCs of the NM group was the highest among the four groups , indicating that the transfection was successful. Among the four groups , β ⁃tubulin Ⅲ mRNA and protein expression was the highest in the NM group , and the GFAP mRNA and protein expression was the lowest. The NI group was the opposite (P < 0. 05) . In the four groups after 24 h , the cell activity of NSCs in the NM group was the strongest and showed a trend of gradually increasing with time (P < 0. 05) . The cell activity of NI was significantly weaker than that of the other three groups (P < 0. 05) . Among the four groups , the apoptosis rate of the NM group was lower than that of the other three groups (P < 0. 05) , and the apoptosis rate of NI was higher than that of the other three groups (P < 0. 05) . The difference in the above indicators between the NO group and the NN group was smaller.
Conclusion:Overexpressed miR⁃9 promotes the proliferation of NSCs by promoting the expression of β ⁃tubulin Ⅲ and inhibiting the expression of GFAP.
- Full text:2024102516482413727miR-9通过调控β-tu...AP对神经干细胞增殖的影响_王煜.pdf
