Distribution characteristics of Mycobacterium tuberculosis and streptomycin and ethambutol resistance patterns by high⁃resolution melt analysis
10.19405/j.cnki.issn1000-1492.2023.07.028
- Author:
Tengfei Guo
1
;
Zhenzhen Wang
1
,
2
;
Yi Hou
3
;
Zhanqin Zhao
4
;
Xiangyang Zu
5
;
Tao Jiang
1
;
Yun Xue
5
Author Information
1. College of Clinical Medicine , Henan University of Science and Technology , Dept of Clinical Laboratory , The First Afiliated Hospital of Henan University of Science and Technology , Luoyang 471000
2. College of Medical Technology and Engineering , Henan University of Science and Technology , Luoyang 471000
3. Luoyang Center for Disease Control and Prevention , Luoyang 471000
4. Animal Science and Technology , Henan University of Science and Technology , Luoyang 471000
5. College of Medical Technology and Engineering , Henan University of Science and Technology , Luoyang 471000
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
resistance patterns;
molecular resistance;
streptomycin;
ethambutol
- From:
Acta Universitatis Medicinalis Anhui
2023;58(7):1227-1232
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To study the resistance pattern of streptomycin and ethambutol in Mycobacterium tuberculosis in Luoyang area , guide clinical medication and supplement epidemiological data on local drug⁃resistant tuberculosis .
Methods :The positive results of high⁃resolution melting curve (HRM) in 2 941 cases in Luoyang area were analyzed to assess the risk factors associated with streptomycin and ethambutol resistance .
Results :Of the 2 941 HRM⁃positive patients , 18 . 4% were resistant to streptomycin and 8. 0% were ethambutol . Both streptomycin and ethambutol and resistance rates were higher in men than those in women ( 19. 0% vs 16. 9% , P = 0. 129 ; 8. 0% vs 7. 9% , P = 0. 987) . The resistance rates to streptomycin and ethambutol were higher in urban than those in rural areas (21 . 3% vs 16. 6% , P = 0. 002 ; 9. 8% vs 6. 9% , P = 0. 004) . The resistance rate was much higher in previously treated patients than those newly diagnosed for MTB infection (25 . 8% vs 17. 3% , P < 0. 001 ; 12. 1% vs 7. 4% , P = 0. 002) . The resistance rates to streptomycin were higher in the < 51 years than those in the > 50 years group (21 . 1% vs 16. 1% , P < 0. 001) . According to age , the highest resistance rates to streptomycin and ethambutol occurred in the age range of 31 - 35 years and 56 - 60 years in men , respectively , while in the age range of 21 - 25 years and 56 - 60 years in women , respectively . In multivariate models , prior treatment history , age less than 51 years , and urban area were positively associated with streptomycin and ethambutol resistance after adjusting for smear results and year testing .
Conclusion :Men , prior treatment history , age less than 51 years , and urban residents are key monitoring targets for streptomycin and ethambutol resistant tuberculosis .
- Full text:2024102417180301396高分辨率熔解曲线检测背景下...霉素和乙胺丁醇耐药模式分析_郭腾飞.pdf
