Protective effects of adipose tissue-derived stromal cells exosomes against pressure-induced injury in retinal ganglion cells of cultured rat in vitro
10.3980/j.issn.1672-5123.2024.11.02
- VernacularTitle:脂肪间充质干细胞外泌体对体外培养压力损伤的大鼠RGCs的保护作用
- Author:
Yidan CHEN
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Min DAI
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Zhikun ZHENG
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Author Information
1. Department of Ophthalmology, Affiliated Hospital of Yunnan University
2. Yunnan Eye Hospital
3. Yunnan Eye Institute
4. Key Laboratory for Prevention and Treatment of Ophthalmic Diseases in Yunnan
5. The Cataract and Retinal Diseases Prevention and Treatment Innovation Team of Yunnan Province Second People's Hospital
6. Yao Ke Expert Workstation in Yunnan Province Clinical Medical Research Center for Ocular Disease in Yunnan
7. Yunnan Clinical Medical Center for Ophthalmic Disease, Kunming 650021, Yunnan Province, China
- Publication Type:Journal Article
- Keywords:
pressure injury;
adipose tissue-derived stromal cells exosomes;
retinal ganglion cells(RGCs);
brain-derived neurotrophic factor;
Caspase-3
- From:
International Eye Science
2024;24(11):1695-1700
- CountryChina
- Language:Chinese
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Abstract:
AIM: To assess the protective effect of adipose tissue-derived mesenchymal stem cells(ADSCs)exosomes on injured retinal ganglion cells(RGCs)by establishing an in vitro rat RGC pressure injury model.METHODS: ADSCs were cultured, and exosomes were extracted from the supernatant and identified. Rat RGCs were divided into a control group, pressure model groups(40, 80, 120 mmHg), and exosome-treated groups under different pressures. Cell proliferation activity was assessed using the CCK-8 assay. The mRNA expression levels of brain-derived neurotrophic factor(BDNF)and Caspase-3 in RGCs were detected by qPCR, and protein levels were measured by Western Blot.RESULTS: The CCK-8 assay showed that cell proliferation activity in the control group increased significantly at 48 h compared to 24 h(P<0.05). At 48 h, cell viability in the exosome-treated groups increased significantly compared to the 40, 80, and 120 mmHg pressure model groups(all P<0.05). qPCR results indicated that BDNF mRNA expression decreased in the 40 mmHg pressure model group without statistical significance(P>0.05), and significantly decreased in the 80 and 120 mmHg pressure model groups(all P<0.05). BDNF mRNA expression significantly increased in the 40 and 80 mmHg pressure model groups after exosome treatment(both P<0.05), and increased in the 120 mmHg pressure model group without statistical significance(P>0.05). Caspase-3 mRNA expression increased in the 40 mmHg pressure model group without statistical significance(P>0.05), and significantly increased in the 80 and 120 mmHg pressure model groups(all P<0.05). Caspase-3 mRNA expression significantly decreased in the 40 and 80 mmHg pressure model groups after exosome treatment(P<0.05), and decreased in the 120 mmHg pressure model group without statistical significance(P>0.05). Western Blot analysis showed that BDNF protein expression decreased in the 40 mmHg pressure model group without statistical significance(P>0.05), and significantly decreased in the 80 and 120 mmHg pressure model groups(all P<0.001). After exosome treatment, BDNF protein expression significantly increased compared to the pressure model groups(all P<0.05). Caspase-3 protein expression increased significantly in all pressure model groups compared to the control group(all P<0.05), and significantly decreased in all exosome-treated groups compared to the model groups(all P<0.05).CONCLUSION: ADSCs-derived exosomes enhance cell proliferation and viability in cultured rat RGCs in vitro under different pressure-induced injuries, enhance BDNF mRNA and protein expression levels, and reduce Caspase-3 mRNA and protein expression levels, suggesting that ADSCs-derived exosomes have a protective effect on pressure-injured in cultured rat RGCs in vitro.
