Study on The Promotion of Tenocyte Proliferation and Differentiation by Oriented Fiber Membrane Loaded With Nano-zinc Oxide
10.16476/j.pibb.2023.0473
- VernacularTitle:载纳米氧化锌定向纤维膜促进肌腱细胞增殖分化的研究
- Author:
Jia FAN
1
;
Peng-Cheng GU
1
;
Xi-Ting CHENG
1
;
Qiang JIANG
1
;
Ya ZHAO
1
;
Xiao-Fang PAN
1
;
Yan BAI
1
Author Information
1. College of Pharmacy, Chongqing Medical University, Chongqing 400016, China
- Publication Type:Journal Article
- Keywords:
nano zinc oxide;
oriented fibers;
tendon repair
- From:
Progress in Biochemistry and Biophysics
2024;51(8):1895-1903
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo simulate the microstructure and mechanical properties of tendon tissue and promote its regeneration and repair, electrospinning technology was used to prepare L-polylactic acid (PLLA) fiber membranes loaded with different nano zinc oxide contents and with oriented structures. Physical and chemical characterization and biological performance evaluation were carried out to explore their effects on tendon cell proliferation and differentiation. MethodsPreparation of PLLA fiber scaffolds and PLLA/ZnO fiber scaffolds containing different mass fractions of nano ZnO was performed using electrospinning technology. The physicochemical properties of the scaffold were characterized by scanning electron microscopy, mechanical stretching, and EDS spectroscopy. The scaffold was co-cultured with mouse tendon cells to detect its biocompatibility and regulatory effects on cell differentiation behavior. ResultsThe fiber scaffolds were arranged in an oriented manner, and zinc elements were uniformly distributed in the fibers. The tensile strength and Young’s modulus of PLLA/0.1%ZnO fiber scaffolds were significantly higher than PLLA fiber scaffolds. The number of cells on the surface of PLLA/0.1%ZnO fiber scaffold was significantly higher than that of the PLLA group, and the activity was better; mouse tendon cells exhibit directional adhesion and growth along the fiber arrangement direction. ConclusionThe oriented PLLA/0.1%ZnO fiber scaffold had excellent physicochemical properties, which can significantly promote the oriented growth, proliferation and differentiation of tendon cells. It is expected to be used for tendon tissue regeneration and repair in the future.
