Target Residence of CRISPR/Cas in Genome Editing
10.16476/j.pibb.2024.0274
- VernacularTitle:“藕断丝连”的CRISPR/Cas：基因编辑中靶点滞留的作用与挑战
- Author:
Yi-Li FENG
1
;
Ruo-Dan CHEN
1
;
An-Yong XIE
1
Author Information
1. Department of General Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310019, China
- Publication Type:Journal Article
- Keywords:
CRISPR/Cas9;
target residence;
target dissociation;
DNA double strand break repair pathway choice;
genome editing heterogeneity
- From:
Progress in Biochemistry and Biophysics
2024;51(10):2621-2636
- CountryChina
- Language:Chinese
-
Abstract:
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is widely used for targeted genomic and epigenomic modifications, transcriptional regulation and real-time cell imaging, and has already demonstrated great potential for applications in agriculture, industry and medicine. The promise of the technology depends upon the five intrinsic properties of CRISPR/Cas: targeting, target unwinding, target cutting, target residence, and collateral cleavage. Here, mainly using Streptococcus pyogenes CRISPR/Cas9 as example, we will focus on the target residence of CRISPR/Cas in applications of the CRISPR/Cas technology, summarize the recent progress, and discuss the effect of CRISPR/Cas target binding and residence on DNA double strand break repair pathway choices and the opportunities that CRISPR/Cas target residence presents to optimize the CRISPR/Cas technology.
